US 11,952,614 B2
Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms
Holger Klapproth, Freiburg (DE); and Nicolaas Smit, Welland (CA)
Assigned to Safeguard Biosystems Holdings Ltd., London (GB)
Filed by SAFEGUARD BIOSYSTEMS HOLDINGS LTD., London (GB)
Filed on Jan. 13, 2021, as Appl. No. 17/147,826.
Application 17/147,826 is a division of application No. 16/023,492, filed on Jun. 29, 2018, abandoned.
Application 16/023,492 is a division of application No. 15/432,157, filed on Feb. 14, 2017, granted, now 10,036,054, issued on Jul. 31, 2018.
Application 15/432,157 is a continuation of application No. PCT/EP2017/051902, filed on Jan. 30, 2017.
Claims priority of application No. 16153540 (EP), filed on Jan. 30, 2016.
Prior Publication US 2021/0139952 A1, May 13, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/68 (2018.01); B01L 3/00 (2006.01); C12M 1/00 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6874 (2018.01); G01N 35/00 (2006.01)
CPC C12Q 1/6806 (2013.01) [B01L 3/5082 (2013.01); C12M 47/06 (2013.01); C12Q 1/6874 (2013.01); B01L 2300/16 (2013.01); G01N 2035/00524 (2013.01)] 28 Claims
 
1. A method for producing a lysate from cells contained in a blood sample, comprising agitating the blood sample within a bead beating system in the absence of a buffer, in the absence of a detergent, and in the presence of one or more endogenous blocking agents comprising urea under conditions sufficient to (i) lyse both a fungal pathogen and a bacterial pathogen and (ii) provide a lysate suitable for PCR amplification of DNA from the fungal pathogen, when present, and the bacterial pathogen, when present, said bead beating system comprising a bead beating tube and beads.