	US 11,952,604 B2
	Enzymatic method for preparing Rebaudioside J
Alex Tao, Jiangsu (CN); Guoqing Li, Jiangsu (CN); Wenxia Wang, Jiangsu (CN); Leilei Zheng, Jiangsu (CN); Chunlei Zhu, Jiangsu (CN); Xiaoliang Liang, Jiangsu (CN); and Kuikiu Chan, Jiangsu (CN)
Assigned to PepsiCo, Inc., Purchase, NY (US)
Filed by PepsiCo, Inc., Purchase, NY (US)
Filed on Jun. 6, 2022, as Appl. No. 17/805,626.
Application 17/805,626 is a continuation of application No. 16/343,340, granted, now 11,359,222, previously published as PCT/CN2016/102942, filed on Oct. 21, 2016.
Prior Publication US 2023/0022453 A1, Jan. 26, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 19/56 (2006.01); C12N 9/10 (2006.01)

CPC C12P 19/56 (2013.01) [C12N 9/1051 (2013.01); C12Y 204/01017 (2013.01)]

12 Claims

1. An enzymatic method for preparing Rebaudioside J, the method comprising reacting Rebaudioside A with a rhamnosyl donor in a reaction system comprising: recombinant cells comprising a UDP-glycosyltransferase, a UDP-glycosyltransferase prepared therefrom, or both the recombinant cells comprising the UDP-glycosyltransferase and the UDP-glycosyltransferase prepared therefrom, wherein the UDP-glycosyltransferase comprises an amino acid sequence having at least 80% sequence identity to SEQ ID NO: 2, wherein Rebaudioside A is converted to Rebaudioside J at a conversion rate greater than 90%.