	US 11,952,598 B2
	Engineered glucosyltransferases
Yougen Li, Pennington, NJ (US); Mark S. Payne, Wilmington, DE (US); Jared B. Parker, Elkton, MD (US); Slavko Kralj, Copenhagen K (DK); Veli Alkan, Palo Alto, CA (US); Richard R. Bott, Palo Alto, CA (US); Robert Dicosimo, Chadds Ford, PA (US); Qiong Cheng, Wilmington, DE (US); Ellen D. Semke, Newark, DE (US); and Susan Marie Hennessey, Avondale, PA (US)
Assigned to NUTRITION & BIOSCIENCES USA 4, INC., Rochester, NY (US)
Filed by NUTRITION & BIOSCIENCES USA 4, INC., Rochester, NY (US)
Filed on Jun. 4, 2021, as Appl. No. 17/338,842.
Application 17/338,842 is a continuation of application No. 16/381,010, filed on Apr. 11, 2019, granted, now 11,028,373.
Application 16/381,010 is a continuation of application No. 15/702,893, filed on Sep. 13, 2017, granted, now 10,301,604.
Claims priority of provisional application 62/394,354, filed on Sep. 14, 2016.
Prior Publication US 2021/0395705 A1, Dec. 23, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/10 (2006.01); C08B 37/00 (2006.01); C12P 19/04 (2006.01); C12P 19/18 (2006.01)

CPC C12N 9/1051 (2013.01) [C08B 37/0009 (2013.01); C12P 19/04 (2013.01); C12P 19/18 (2013.01)]

20 Claims

1. A non-native glucosyltransferase comprising an amino acid substitution at a position corresponding with amino acid residue Asp-948 of SEQ ID NO:62,

wherein the non-native glucosyltransferase synthesizes alpha-glucan comprising 1,3-linkages, and

wherein the non-native glucosyltransferase has:

(i) an alpha-glucan yield that is higher than the alpha-glucan yield of a second glucosyltransferase that only differs from the non-native glucosyltransferase at the substitution position, and/or

(ii) a leucrose yield that is lower than the leucrose yield of the second glucosyltransferase,

wherein the non-native glucosyltransferase comprises a catalytic domain that is at least about 90% identical to residues 55-960 of SEQ ID NO:4, residues 54-957 of SEQ ID NO:65, residues 55-960 of SEQ ID NO:30, residues 55-960 of SEQ ID NO:28, or residues 55-960 of SEQ ID NO:20.