US 11,952,595 B2
Production of lytic phages
Jesus Fernandez Rodriguez, Paris (FR); Antoine Decrulle, Paris (FR); Aymeric Leveau, Paris (FR); Ines Canadas Blasco, Paris (FR); Aurelie Mathieu, Paris (FR); and Thibault Carlier, Paris (FR)
Assigned to Eligo Bioscience, Paris (FR)
Filed by Eligo Bioscience, Paris (FR)
Filed on Jul. 7, 2023, as Appl. No. 18/348,910.
Application 18/348,910 is a division of application No. 17/742,693, filed on May 12, 2022, granted, now 11,739,304.
Claims priority of provisional application 63/187,532, filed on May 12, 2021.
Claims priority of provisional application 63/187,531, filed on May 12, 2021.
Prior Publication US 2024/0010994 A1, Jan. 11, 2024
Int. Cl. C12N 7/00 (2006.01); C12N 1/20 (2006.01); C12N 15/70 (2006.01); C12N 15/74 (2006.01)
CPC C12N 7/00 (2013.01) [C12N 1/20 (2013.01); C12N 15/70 (2013.01); C12N 15/74 (2013.01); C12N 2795/10322 (2013.01); C12N 2795/10352 (2013.01); C12N 2800/101 (2013.01); C12N 2800/40 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A method for producing lytic phage particles or lytic phage-derived delivery vehicles, comprising:
(a) providing a production bacterial cell stably comprising phage structural genes and phage DNA packaging genes of a lytic bacteriophage,
wherein the expression of said phage structural genes and phage DNA packaging genes in said production bacterial cell is controlled by an induction mechanism comprising phage excision/insertion genes, phage DNA replication genes, and phage regulation genes of a second, non-lytic bacteriophage, wherein said phage excision/insertion genes, phage DNA replication genes and phage regulation genes are neither phage DNA packaging genes nor phage structural genes,
wherein said production bacterial cell does not comprise phage excision/insertion genes and/or phage replication genes of the lytic bacteriophage,
wherein said production bacterial cell is a P. freudenreichii bacterial cell,
wherein the lytic bacteriophage is a C acnes phage and
wherein the second, non-lytic bacteriophage is a P. freudenreichii phage; and
(b) inducing, in said production bacterial cell, expression of said phage structural genes and phage DNA packaging genes, and assembly of the products expressed by said phage structural genes and phage DNA packaging genes, thereby producing lytic phage particles or lytic phage-derived delivery vehicles.