	US 11,952,587 B2
	Method for producing platelets
Koji Eto, Kyoto (JP); Sou Nakamura, Kyoto (JP); and Yukitaka Ito, Kyoto (JP)
Assigned to KYOTO UNIVERSITY, Kyoto (JP); and MEGAKARYON CORPORATION, Kyoto (JP)
Appl. No. 16/491,219
Filed by KYOTO UNIVERSITY, Kyoto (JP); and MEGAKARYON CORPORATION, Kyoto (JP)
PCT Filed Mar. 5, 2018, PCT No. PCT/JP2018/008279 § 371(c)(1), (2) Date Jan. 15, 2020, PCT Pub. No. WO2018/164040, PCT Pub. Date Sep. 13, 2018.
Claims priority of application No. 2017-042033 (JP), filed on Mar. 6, 2017; and application No. 2017-089855 (JP), filed on Apr. 28, 2017.
Prior Publication US 2020/0216807 A1, Jul. 9, 2020
Int. Cl. C12N 5/078 (2010.01); C12N 15/85 (2006.01)

CPC C12N 5/0644 (2013.01) [C12N 15/85 (2013.01); C12N 2501/105 (2013.01); C12N 2506/02 (2013.01)]

8 Claims

1. A method for producing platelets, comprising the steps of:

(1) culturing megakaryocytes in a platelet producing medium, and

(2) harvesting platelets obtained by the culturing;

wherein the step of culturing comprises:

(a) promoting a release of one or more platelet production promoting factor(s), wherein the factor(s) comprise MIF, NRDc, IGFBP2, TSP-1, PAI-1, or CCL5, from megakaryocytes by applying mechanical stress including turbulence by vortex; and/or

(b) externally adding platelet production promoting factors including MIF, NRDc, and IGFBP2,

wherein the step of culturing comprises step (b) and wherein step (b) further comprises externally adding at least one platelet production promoting factor(s) comprising TSP-1, PAI-1, or CCL5.