US 11,952,581 B2
Process for the production of polyunsaturated fatty acids in transgenic organisms
Thorsten Zank, Mannheim (DE); Jörg Bauer, Research Triangle Park, NC (US); Petra Cirpus, Mannheim (DE); Amine Abbadi, Ebergötzen (DE); Ernst Heinz, Hamburg (DE); Xiao Qiu, Saskatoon (CA); Patricia Vrinten, Saskatoon (CA); Petra Sperling, Hamburg (DE); Frederic Domergue, Hamburg (DE); Astrid Schmidt, Hamburg (DE); and Helene Kirsch, Hamburg (DE)
Assigned to BASF PLANT SCIENCE GMBH, Ludwigshafen (DE)
Filed by BASF PLANT SCIENCE GMBH, Ludwigshafen (DE)
Filed on Nov. 22, 2021, as Appl. No. 17/532,634.
Application 15/237,901 is a division of application No. 10/566,944, granted, now 9,433,228, previously published as PCT/EP2004/007957, filed on Jul. 16, 2004.
Application 17/532,634 is a continuation of application No. 16/509,247, filed on Jul. 11, 2019, granted, now 11,180,769.
Application 16/509,247 is a continuation of application No. 15/237,901, filed on Aug. 16, 2016, abandoned.
Claims priority of application No. 10335992.3 (DE), filed on Aug. 1, 2003; application No. 10344557.9 (DE), filed on Sep. 24, 2003; application No. 10347869.8 (DE), filed on Oct. 10, 2003; application No. 10359593.7 (DE), filed on Dec. 18, 2003; application No. 102004009457.8 (DE), filed on Feb. 27, 2004; application No. 102004012370.5 (DE), filed on Mar. 13, 2004; and application No. 102004024014.0 (DE), filed on May 14, 2004.
Prior Publication US 2022/0162629 A1, May 26, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. A01H 1/00 (2006.01); A23D 9/00 (2006.01); A23D 9/02 (2006.01); A23L 33/115 (2016.01); C11B 1/00 (2006.01); C12N 9/02 (2006.01); C12N 9/10 (2006.01); C12N 15/82 (2006.01); C12P 7/64 (2022.01); C12P 7/6409 (2022.01); C12P 7/6427 (2022.01); C12P 7/6463 (2022.01); C12P 7/6472 (2022.01)
CPC C12N 15/8247 (2013.01) [A01H 1/00 (2013.01); A23D 9/00 (2013.01); A23D 9/02 (2013.01); A23L 33/115 (2016.08); C11B 1/00 (2013.01); C12N 9/0071 (2013.01); C12N 9/0083 (2013.01); C12N 9/0093 (2013.01); C12N 9/1029 (2013.01); C12P 7/6409 (2013.01); C12P 7/6427 (2013.01); C12P 7/6463 (2013.01); C12P 7/6472 (2013.01); C12Y 114/19 (2013.01); C12Y 114/19003 (2013.01); C12Y 203/01199 (2015.07); A23V 2002/00 (2013.01); Y02P 20/52 (2015.11)] 6 Claims
 
1. A process for producing docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), said process comprising:
growing a transgenic plant comprising:
a) least one nucleic acid sequence which encodes a polypeptide with Δ6-desaturase activity, and
b) at least one nucleic acid sequence which encodes a polypeptide with Δ6-elongase activity, and
c) at least one nucleic acid sequence which encodes a polypeptide with Δ5-desaturase activity, and
d) at least one nucleic acid sequence which encodes a polypeptide with Δ5-elongase activity, and
e) at least one nucleic acid sequence which encodes a polypeptide with Δ4-desaturase activity, and
wherein the Δ6-desaturase uses CoA fatty acid esters as a substrate,
wherein the at least one nucleic acid encoding a polypeptide with Δ6-desaturase activity comprises:
i) the nucleotide sequence of SEQ ID NO: 89;
ii) a nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 90; or
iii) a nucleotide sequence encoding a polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 90, and
harvesting the transgenic plant.