US 11,952,580 B2
Heterologous production of psilocybin
Dominik Mojzita, Espoo (FI); Heiko Rischer, Espoo (FI); Anssi Rantasalo, Espoo (FI); Kirsi-Marja Oksman-Caldentey, Espoo (FI); and Joosu Kuivanen, Espoo (FI)
Assigned to TEKNOLOGIAN TUTKIMUSKESKUS VTT OY, Espoo (FI)
Appl. No. 16/980,710
Filed by TEKNOLOGIAN TUTKIMUSKESKUS VTT OY, Espoo (FI)
PCT Filed Mar. 11, 2019, PCT No. PCT/FI2019/050199
§ 371(c)(1), (2) Date Sep. 14, 2020,
PCT Pub. No. WO2019/180309, PCT Pub. Date Sep. 26, 2019.
Claims priority of application No. 20185254 (FI), filed on Mar. 19, 2018.
Prior Publication US 2021/0010015 A1, Jan. 14, 2021
Int. Cl. C12N 15/82 (2006.01); C12N 9/10 (2006.01); C12N 15/52 (2006.01)
CPC C12N 15/8243 (2013.01) [C12N 9/1007 (2013.01); C12N 15/52 (2013.01)] 11 Claims
 
1. A recombinant plant host cell comprising:
heterologous polynucleotides encoding PsiD, PsiH, PsiK, and PsiM,
wherein the heterologous polynucleotides are operably linked to at least one promoter which is capable of providing constitutive expression of said heterologous polynucleotides in the recombinant plant host cell;
wherein PsiD has at least 80% amino acid sequence identity with the protein sequence deposited in GenBank accession number ASU62239.1 or the GenBank accession number ASU62242.1, or with the amino acid sequence encoded by polynucleotide SEQ ID NO: 1 or 2 or 9 or 10;
wherein PsiH has at least 80% amino acid sequence identity with the protein sequence deposited in GenBank accession number ASU62246.1 or the GenBank accession number ASU62250.1, or with the amino acid sequence encoded by polynucleotide SEQ ID NO: 5 or 6 or 13 or 14;
wherein PsiK has at least 80% amino acid sequence identity with the protein sequence deposited in GenBank accession number ASU62237.1 or the GenBank accession number ASU62240.1, or with the amino acid sequence encoded by polynucleotide SEQ ID NO: 7 or 8 or 15 or 16;
wherein PsiM has at least 80% amino acid sequence identity with the protein sequence deposited in GenBank accession number ASU62238.1 or the GenBank accession number ASU62241.1, or with the amino acid sequence encoded by polynucleotide SEQ ID NO: 3 or 4 or 11 or 12; and
wherein the recombinant plant host cell is capable of producing psilocybin.