US 11,952,579 B2
Interfering with HD-Zip transcription factor repression of gene expression to produce plants with enhanced traits
Cara L. Griffith, Catawissa, MO (US); Abha Khandelwal, Chesterfield, MO (US); Paul J. Loida, Kirkwood, MO (US); Elena A. Rice, Olivette, MO (US); Rebecca L. Thompson, St. Charles, MO (US); and Sivalinganna Manjunath, Chesterfield, MO (US)
Assigned to Monsanto Technology LLC, St. Louis, MO (US)
Filed by Monsanto Technology LLC, St. Louis, MO (US)
Filed on Oct. 15, 2020, as Appl. No. 17/071,231.
Application 17/071,231 is a division of application No. 15/028,381, granted, now 10,829,773, previously published as PCT/US2014/058594, filed on Oct. 1, 2014.
Claims priority of provisional application 61/888,980, filed on Oct. 9, 2013.
Prior Publication US 2021/0095300 A1, Apr. 1, 2021
Int. Cl. C12N 15/82 (2006.01)
CPC C12N 15/8216 (2013.01) 8 Claims
 
1. A recombinant DNA construct comprising a DNA molecule that encodes a N-terminal truncation variant of a HD-Zip class II transcription factor protein that has an amino acid sequence with at least 95% amino acid sequence identity with the full-length amino acid sequence of SEQ ID NO: 113, and operably linked to a heterologous promoter, wherein the N-terminal truncation variant is missing the N-terminal amino acid portion comprising amino acid position 1 to amino acid position 59 of the HD-Zip class II amino acid sequence transcription factor protein relative to the amino acid sequence of SEQ ID NO: 26, and wherein said N-terminal truncation variant of the HD-Zip class II transcription factor protein has a loss-of-function mutation in a transcriptional repression domain.