	US 11,952,558 B2
	Subtilase variants
Miguel Duarte Guilherme Pereira Toscano, Greve (DK); Esben Peter Friis, Herlev (DK); Rolf Thomas Lenhard, Lyngby (DK); Signe Eskildsen Larsen, Kgs. Lyngby (DK); Astrid Munch, Frederiksberg (DK); Bena-Marie Lue, Holte (DK); and Mikael Bauer, Malmo (SE)
Assigned to NOVOZYMES A/S, Bagsvaerd (DK)
Filed by NOVOZYMES A/S, Bagsvaerd (DK)
Filed on Apr. 6, 2021, as Appl. No. 17/223,928.
Application 17/223,928 is a division of application No. 15/534,522, granted, now 10,995,304, previously published as PCT/EP2015/079574, filed on Dec. 14, 2015.
Claims priority of application No. 14197966 (EP), filed on Dec. 15, 2014; and application No. 14197968 (EP), filed on Dec. 15, 2014.
Prior Publication US 2021/0230516 A1, Jul. 29, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/54 (2006.01); C11D 3/386 (2006.01)

CPC C11D 3/38618 (2013.01) [C12N 9/54 (2013.01); C12Y 304/21112 (2013.01)]

16 Claims

1. A subtilase variant having at least 90% sequence identity to SEQ ID NO: 3 wherein the variant has a glutamic acid residue (E) in position 101, wherein the subtilase variant further comprises one or more substitutions selected from the group consisting of S3T, R45E,D,Q, P55N, T58W,Y,L, Q59D,M,N,T, G61D,R, S87E, G97S, A98D,E,R, S106A,W, N117E, H120V,D,K,N, S124M, P129D, E136Q, Q137H, S143W, S156D, S161T, S163A,G, Y171L, A172S, N185Q, V199M, Y209W, M222Q, N238H, V244T, N261T,D and L262N,Q,D,E, wherein the variant has increased stability in a liquid detergent composition compared to the subtilase having the amino acid sequence of SEQ ID NO: 3 and wherein the positions corresponds to the positions of SEQ ID NO: 1.