US 11,952,468 B2
Metabolic enzyme-induced micro-nano plastic particle biodegradation method and product analysis method therefor
Qian Liu, Beijing (CN); Xiu Huang, Beijing (CN); and Guibin Jiang, Beijing (CN)
Assigned to RESEARCH CENTER FOR ECO-ENVIRONMENTAL SCIENCES, CHINESE ACADEMY OF SCIENCES, Beijing (CN)
Appl. No. 18/274,244
Filed by RESEARCH CENTER FOR ECO-ENVIRONMENTAL SCIENCES, CHINESE ACADEMY OF SCIENCES, Beijing (CN)
PCT Filed Nov. 4, 2021, PCT No. PCT/CN2021/128825
§ 371(c)(1), (2) Date Jul. 26, 2023,
PCT Pub. No. WO2022/160841, PCT Pub. Date Aug. 4, 2022.
Claims priority of application No. 202110114200.X (CN), filed on Jan. 27, 2021.
Prior Publication US 2024/0043650 A1, Feb. 8, 2024
Int. Cl. C08J 11/10 (2006.01); C08J 3/12 (2006.01); C12N 9/10 (2006.01); G01N 1/40 (2006.01); G01N 27/623 (2021.01)
CPC C08J 11/105 (2013.01) [C08J 3/12 (2013.01); C12N 9/1088 (2013.01); G01N 1/4022 (2013.01); G01N 27/623 (2021.01); C08J 2400/20 (2013.01); G01N 2001/4027 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A metabolic enzyme-induced micro-nano plastic particle biodegradation method, comprising the following steps:
step (1), cutting an appropriate quantity of plastic products into small pieces;
step (2), placing the plastic products in a ball mill tank and milling the plastic products in a vacuum in a ball mill;
step (3), weighing and dispersing milled plastic products with water to prepare a micro-nano plastic dispersion with a concentration;
step (4), mixing the micro-nano plastic dispersion with a metabolic enzyme solution in a mass ratio range of a metabolic enzyme mixed with micro-nano plastics of 0.00000001-10000, and placing a mixed solution in a vortex oscillator for mixing well, wherein the metabolic enzyme comprises glutathione S-transferase; and
step (5), incubating the mixed solution in a waterproof incubator.