US 11,952,408 B2
HPV-specific binding molecules
Cameron Brandt, Seattle, WA (US); Brian Belmont, Brookline, MA (US); Christopher Borges, Cambridge, MA (US); Stephen Michael Burleigh, Seattle, WA (US); Alexandra Croft, Seattle, WA (US); Stephen Jacob Goldfless, Seattle, WA (US); David Jeffrey Huss, Seattle, WA (US); Yue Jiang, Seattle, WA (US); Timothy G. Johnstone, Seattle, WA (US); David Koppstein, Seattle, WA (US); Hieu Nguyen, Seattle, WA (US); Christopher Heath Nye, Seattle, WA (US); Haley Peper, Seattle, WA (US); Blythe D. Sather, Seattle, WA (US); Sonia Timberlake, Seattle, WA (US); Dean Y. Toy, Seattle, WA (US); Queenie Vong, Seattle, WA (US); Gordon Grant Welstead, Cambridge, MA (US); and James Sissons, Seattle, WA (US)
Assigned to Juno Therapeutics, Inc., Seattle, WA (US); and Editas Medicine, Inc., Cambridge, MA (US)
Appl. No. 16/652,379
Filed by Juno Therapeutics, Inc., Seattle, WA (US); and Editas Medicine, Inc., Cambridge, MA (US)
PCT Filed Sep. 28, 2018, PCT No. PCT/US2018/053650
§ 371(c)(1), (2) Date Mar. 30, 2020,
PCT Pub. No. WO2019/070541, PCT Pub. Date Apr. 11, 2019.
Claims priority of provisional application 62/653,529, filed on Apr. 5, 2018.
Claims priority of provisional application 62/597,411, filed on Dec. 11, 2017.
Claims priority of provisional application 62/567,750, filed on Oct. 3, 2017.
Prior Publication US 2021/0284709 A1, Sep. 16, 2021
Int. Cl. C07K 14/705 (2006.01); A61K 35/17 (2015.01); A61P 31/20 (2006.01); A61P 35/00 (2006.01); C07K 14/725 (2006.01); C12N 9/22 (2006.01); C12N 15/11 (2006.01); C12N 15/90 (2006.01)
CPC C07K 14/7051 (2013.01) [A61K 35/17 (2013.01); A61P 31/20 (2018.01); A61P 35/00 (2018.01); C12N 9/22 (2013.01); C12N 15/11 (2013.01); C12N 15/907 (2013.01); C12N 2310/20 (2017.05); C12N 2800/80 (2013.01)] 46 Claims
 
1. A polynucleotide, comprising:
(a) a nucleic acid sequence encoding a T cell receptor (TCR) or an antigen-binding portion thereof that binds to or recognizes a peptide epitope of human papillomavirus (HPV) 16 in the context of an MHC molecule, and
(b) two or more homology arm(s) linked to the nucleic acid sequence, wherein: the two or more homology arms comprise a sequence homologous to one or more region(s) of an open reading frame of a T cell receptor alpha constant (TRAC) locus;
wherein the two or more homology arm(s) comprises a 5′ homology arm and a 3′ homology arm,
wherein the 5′ homology arm comprises the sequence set forth in SEQ ID NO: 1343, and
wherein the 3′ homology arm comprises a sequence comprising at least 150 contiguous nucleotides of the sequence set forth in SEQ ID NO: 1344.