US 11,952,401 B2
Recombinant foot-and-mouth disease virus with reduced immunosuppression activity, and preparation method and use thereof
Haixue Zheng, Lanzhou (CN); Fan Yang, Lanzhou (CN); Zixiang Zhu, Lanzhou (CN); Xiangle Zhang, Lanzhou (CN); Weijun Cao, Lanzhou (CN); Congcong Wang, Lanzhou (CN); Kangli Li, Lanzhou (CN); Huisheng Liu, Lanzhou (CN); Hong Tian, Lanzhou (CN); Keshan Zhang, Lanzhou (CN); and Xiangtao Liu, Lanzhou (CN)
Assigned to Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou (CN)
Filed by Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou (CN)
Filed on Jul. 14, 2021, as Appl. No. 17/375,203.
Claims priority of application No. 202010676834.X (CN), filed on Jul. 14, 2020.
Prior Publication US 2022/0017576 A1, Jan. 20, 2022
Int. Cl. C07K 14/005 (2006.01); A61K 39/00 (2006.01); A61K 39/135 (2006.01); A61P 31/14 (2006.01); A61P 37/06 (2006.01); C12N 7/00 (2006.01)
CPC C07K 14/005 (2013.01) [A61K 39/135 (2013.01); A61P 31/14 (2018.01); A61P 37/06 (2018.01); C12N 7/00 (2013.01); A61K 2039/525 (2013.01); A61K 2039/552 (2013.01); C12N 2770/32121 (2013.01); C12N 2770/32122 (2013.01); C12N 2770/32134 (2013.01); C12N 2770/32152 (2013.01); C12N 2770/32171 (2013.01)] 13 Claims
 
1. A method for preparing a recombinant foot-and-mouth disease virus (FMDV), comprising mutating the amino acid at position 17 for each of 3B1, 3B2, and 3B3 of an FMDV 3B protein to glutamate to eliminate the immunosuppressive function of the FMDV 3B protein.