	US 11,952,398 B2
	Chromatographic method for collecting blood coagulation factor VII with high yield
Kentaro Ebisu, Koshi (JP); Takashi Kai, Koshi (JP); and Nozomi Matsuo, Kumamoto (JP)
Assigned to KM Biologics Co., Ltd., Kumamoto (JP)
Filed by KM Biologics Co., Ltd., Kumamoto (JP)
Filed on Jun. 9, 2021, as Appl. No. 17/342,928.
Application 17/342,928 is a division of application No. 16/471,830, granted, now 11,059,857, previously published as PCT/JP2017/045680, filed on Dec. 20, 2017.
Claims priority of application No. 2016-249815 (JP), filed on Dec. 22, 2016.
Prior Publication US 2021/0292359 A1, Sep. 23, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 1/18 (2006.01); A61K 38/36 (2006.01); C07K 1/22 (2006.01)

CPC C07K 1/18 (2013.01) [A61K 38/36 (2013.01)]

5 Claims

1. A method for purifying blood coagulation factor VII (Factor VII) and activated blood coagulation factor VII (activated Factor VII) from cryoprecipitate-poor plasma-derived fraction, comprising the following steps:

(a) applying cryoprecipitate-poor plasma-derived fraction to a first strong anion exchange resin;

(b) adsorbing Factor VII and activated Factor VII from the cryoprecipitate-poor plasma-derived fraction to the first strong anion exchange resin and recovering a non-adsorbed fraction;

(c) adsorbing Factor VII and activated Factor VII which remains in the non-adsorbed fraction in step (b) to a second strong anion exchange resin; and

(d) recovering Factor VII and activated Factor VII from first and second strong anion exchange resins in steps (b) and (c), by elution wherein recovering Factor VII and activated Factor VII from the first strong anion exchange resin and second strong anion exchange resin occurs simultaneously.