	US 11,951,479 B2
	Microfluidic system, method for inhibiting, delaying, or reversing cellular senescence using microfluidic system, and cell obtained therefrom
Ssang-Goo Cho, Seoul (KR); Aram Chung, Seoul (KR); Kyung Min Lim, Seoul (KR); Geunho Kang, Anyang-si (KR); and Soobin Jang, Daegu (KR)
Assigned to KONKUK UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION, Seoul (KR); and KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION, Seoul (KR)
Filed by Konkuk University Industry-Academic Cooperation Foundation, Seoul (KR); and Korea University Research and Business Foundation, Seoul (KR)
Filed on Jul. 29, 2021, as Appl. No. 17/389,138.
Claims priority of application No. 10-2020-0094616 (KR), filed on Jul. 29, 2020; and application No. 10-2021-0098400 (KR), filed on Jul. 27, 2021.
Prior Publication US 2022/0032304 A1, Feb. 3, 2022
Int. Cl. B01L 3/00 (2006.01); C12N 5/0775 (2010.01)

CPC B01L 3/502746 (2013.01) [C12N 5/0665 (2013.01); B01L 2400/0478 (2013.01)]

10 Claims

1. A method for inhibiting, delaying, or reversing cellular senescence, comprising:

having an isolated cell flow; and

crashing the cell into an impact surface installed on a flow path of the cell to apply a physical impact to the cell, whereby a reorganization of cytoskeleton occurs in the cell without causing cell death,

wherein the having an isolated cell flow is performed by injecting a fluid containing the cell into a microchannel to flow in accordance with a predetermined parameter (Re),

wherein the parameter (Re) is determined by Equation 1:

Re=ρVD/μ (Equation 1)

wherein, in Equation 1, ρ is a density of the fluid, V is a velocity of the fluid, D is a characteristic length of the fluid, and μ is a viscosity coefficient of the fluid, and

wherein the parameter (Re) has a value of from 75 to 290.