US 11,951,417 B2
SEC performance enhancing conditioning and storage solvents containing low levels of buffer and salt
Pamela C. Iraneta, Brighton, MA (US); Stephen James Shiner, Holden, MA (US); Raymond P. Fisk, Norton, MA (US); and Lavelay Kizekai, Coventry, RI (US)
Assigned to Waters Technologies Corporation, Milford, MA (US)
Filed by Waters Technologies Corporation, Milford, MA (US)
Filed on Apr. 15, 2021, as Appl. No. 17/231,808.
Claims priority of provisional application 63/010,487, filed on Apr. 15, 2020.
Prior Publication US 2021/0322896 A1, Oct. 21, 2021
Int. Cl. B01D 15/20 (2006.01); B01D 15/34 (2006.01); B01D 71/06 (2006.01); G01N 30/50 (2006.01)
CPC B01D 15/20 (2013.01) [B01D 15/34 (2013.01); B01D 71/06 (2013.01); G01N 30/50 (2013.01)] 9 Claims
OG exemplary drawing
 
1. A storage solvent for long-term storage of a column comprising porous particles comprising a silicon-based inorganic-organic hybrid material for performing protein-based size exclusion chromatography, the storage solvent comprising water, from 5 to 15 vol % acetonitrile, from 5 to 100 mM of an alkali metal phosphate salt, and from 50 to 200 mM potassium chloride, wherein the storage solvent is for storing the column for a period of at least two weeks while providing a start peak-height to valley-height ratio (p/v) greater than 1.3.