US 11,951,233 B2
Acellular organs, and methods of producing the same
Dar-Jen Hsieh, Kaohsiung (TW); Chao-Yi Wei, Kaohsiung (TW); Chao-Chin Chao, Kaohsiung (TW); Jer-Cheng Kuo, Kaohsiung (TW); Yi-Ping Lai, Kaohsiung (TW); and Srinivasan Periasamy, Kaohsiung (TW)
Assigned to ACRO BIOMEDICAL COMPANY. LTD., Kaohsiung (TW)
Appl. No. 17/251,790
Filed by ACRO BIOMEDICAL COMPANY. LTD., Kaohsiung (TW)
PCT Filed Sep. 11, 2019, PCT No. PCT/CN2019/105300
§ 371(c)(1), (2) Date Dec. 12, 2020,
PCT Pub. No. WO2020/052580, PCT Pub. Date Mar. 19, 2020.
Claims priority of provisional application 62/729,983, filed on Sep. 11, 2018.
Prior Publication US 2021/0205497 A1, Jul. 8, 2021
Int. Cl. A61K 36/00 (2006.01); A61L 27/36 (2006.01)
CPC A61L 27/3691 (2013.01) [A61L 27/3625 (2013.01); A61L 27/3687 (2013.01); A61L 2430/20 (2013.01); A61L 2430/40 (2013.01)] 18 Claims
 
1. A method of producing an acellular organ, comprising:
(a) subjecting an organ to a first supercritical fluid (SCF) in the presence of a first co-solvent under a first pressure of 200-500 bar at a first temperature between 30-50° C. for a first period of 10-100 minutes; and
(b) subjecting the first SCF treated organ of the step (a) with a continuous flow of a second SCF in the presence of a second co-solvent under a second pressure of 200-500 bar at a second temperature between 30-50° C. for a second period of 10-100 minutes, wherein the flow rate of the second SCF is 10-30 liter per minute;
wherein, the method does not comprise the step of treating the organ with an agent selected from the group consisting of an enzyme, an ion chelating agent, a detergent, a glycerol, and a combination thereof.