US 11,951,211 B2
DNA brick-assisted liposome sorting
Chenxiang Lin, Orange, CT (US); and Yang Yang, Watertown, MA (US)
Assigned to YALE UNIVERSITY, New Haven, CT (US)
Filed by Yale University, New Haven, CT (US)
Filed on Jan. 29, 2021, as Appl. No. 17/162,008.
Claims priority of provisional application 62/968,683, filed on Jan. 31, 2020.
Prior Publication US 2021/0267894 A1, Sep. 2, 2021
Int. Cl. A61K 9/127 (2006.01); A61K 47/69 (2017.01)
CPC A61K 9/1272 (2013.01) [A61K 9/1277 (2013.01); A61K 47/6913 (2017.08)] 18 Claims
OG exemplary drawing
 
1. A method for producing uniform-size liposomes, the method comprising:
coating a plurality of liposomes with a sorting agent to yield a plurality of density-modified liposomes of different sizes;
separating the density-modified liposomes of different sizes using a densitometric method; and
isolating one or more fractions of uniform-size liposomes,
wherein the sorting agent comprises a density-modifying moiety and a targeting moiety, wherein the molecular mass of the density-modifying moiety is from 50 to 250 kDa, and
wherein the liposomes within each of the one or more isolated fractions have a coefficient of variation of less than 15%.