US 11,951,210 B2
Methods for making giant vesicles and their use
Shiva Razavi, Baltimore, MD (US); Takanari Inoue, Lutherville-Timonium, MD (US); Tianzhi Luo, Anhui (CN); and Douglas Robinson, Lutherville-Timonium, MD (US)
Assigned to THE JOHNS HOPKINS UNIVERSTY, Baltimore, MD (US)
Appl. No. 16/772,518
Filed by THE JOHNS HOPKINS UNIVERSITY, Baltimore, MD (US)
PCT Filed Dec. 12, 2018, PCT No. PCT/US2018/065237
§ 371(c)(1), (2) Date Jun. 12, 2020,
PCT Pub. No. WO2019/118621, PCT Pub. Date Jun. 20, 2019.
Claims priority of provisional application 62/597,631, filed on Dec. 12, 2017.
Prior Publication US 2021/0145746 A1, May 20, 2021
Int. Cl. A61K 9/127 (2006.01)
CPC A61K 9/1271 (2013.01) [A61K 9/1277 (2013.01)] 5 Claims
OG exemplary drawing
 
1. A giant unilamellar vesicle (GUV) comprising a unilamellar lipid bilayer, wherein the unilamellar lipid bilayer includes the outer surface of the GUV and encloses the inner lumen of the GUV;
wherein the unilamellar lipid bilayer comprises at least a first lipid and second lipid selected from a phosphatidylcholine (PC), phosphatidylserine (PS), phosphatidylglycerol (PG), phosphatidylinositol 4,5-biphosphate (PIP2), and DGS-NTA (Ni), and combinations thereof wherein the first and second lipid and combinations thereof may be the same or different;
and wherein the GUV comprises
a chemically inducible dimerization protein in the inner lumen and a binding partner protein on the outer surface; wherein the binding partner protein on the outer surface is fused with myristoylated alanine-rich protein kinase C substrate (MARCKS) or a C2 domain of lactadherin (lact-C2).