	US 11,951,168 B2
	Composition containing buffer
Yuichi Funase, Ashigarakami-gun (JP); and Masahiro Kurokawa, Sammu (JP)
Assigned to PERSEUS PROTEOMICS INC., Tokyo (JP)
Appl. No. 16/321,973
Filed by PERSEUS PROTEOMICS INC., Tokyo (JP)
PCT Filed Aug. 21, 2017, PCT No. PCT/JP2017/029696 § 371(c)(1), (2) Date Jan. 30, 2019, PCT Pub. No. WO2018/034354, PCT Pub. Date Feb. 22, 2018.
Claims priority of application No. 2016-161114 (JP), filed on Aug. 19, 2016.
Prior Publication US 2019/0175736 A1, Jun. 13, 2019
Int. Cl. A61K 47/12 (2006.01); A61K 33/24 (2019.01); A61K 33/244 (2019.01); A61K 47/18 (2017.01); A61K 47/50 (2017.01); A61K 51/00 (2006.01); A61K 51/04 (2006.01); A61P 35/00 (2006.01); A61P 35/02 (2006.01); A61K 9/00 (2006.01); A61K 51/12 (2006.01)

CPC A61K 47/12 (2013.01) [A61K 33/24 (2013.01); A61K 33/244 (2019.01); A61K 47/18 (2013.01); A61K 47/50 (2017.08); A61K 51/00 (2013.01); A61K 51/048 (2013.01); A61K 51/0482 (2013.01); A61P 35/00 (2018.01); A61P 35/02 (2018.01); A61K 9/0019 (2013.01); A61K 51/1241 (2013.01)]

8 Claims

1. A method of producing a radioactive metal-labeled chelated targeting agent, the method comprising:

adding a radioactive metal selected from the group consisting of ⁹⁰Y, ¹⁵³Sm, ¹⁶⁵Dy, ¹⁶⁵Er, ¹⁶⁶Ho, and ¹⁷⁷Lu to a mixture of a chelated targeting agent selected from the group consisting of a low-molecular-weight compound, a peptide, a protein, an antibody, an antibody fragment, and a nucleic acid chelated with at least one member selected from the group consisting of

and at least one buffer selected from the group consisting of benzoic acid, maleic acid, and salts thereof to form a complex between the radioactive metal and the chelated targeting agent,

wherein a pH of a reaction liquid at the time of labelling of the chelated targeting agent with the radioactive metal is adjusted to fall within a range of the pKa value of the buffer plus or minus 1.5.