US 12,270,808 B2
Paramagnetic immunobeads for the isolation of human adipose-derived stem cells
John D. Murray, Jacksonville, FL (US); and Edward W. Scott, Gainesville, FL (US)
Assigned to University of Florida Research Foundation, Inc., Gainesville, FL (US)
Appl. No. 17/056,889
Filed by University of Florida Research Foundation, Inc., Gainesville, FL (US)
PCT Filed May 21, 2019, PCT No. PCT/US2019/033208
§ 371(c)(1), (2) Date Nov. 19, 2020,
PCT Pub. No. WO2019/226588, PCT Pub. Date Nov. 28, 2019.
Claims priority of provisional application 62/675,298, filed on May 23, 2018.
Prior Publication US 2021/0208137 A1, Jul. 8, 2021
Int. Cl. G01N 33/543 (2006.01); B03C 1/01 (2006.01); B03C 1/28 (2006.01)
CPC G01N 33/54326 (2013.01) [B03C 1/01 (2013.01); B03C 1/28 (2013.01); B03C 2201/18 (2013.01); B03C 2201/26 (2013.01)] 5 Claims
 
1. A paramagnetic immunobead (PIB) for isolating human adipose-tissue derived stem cells, consisting of a magnetic material displaying on its surface a first antibody that selectively binds human CD90, a second antibody that selectively binds human CD44, a third antibody that selectively binds human CD105, and a fourth antibody that selectively binds human CD73, wherein the magnetic material is a paramagnetic spherical polymer particle.