US 12,270,783 B2
Single cell gel electrophoresis
Oliver Schicht, Baar (CH); and Arne-Christian Faisst, Oberdorf (CH)
Assigned to 4D LIFETEC AG, Cham (CH)
Appl. No. 16/960,124
Filed by 4D Lifetec AG, Cham (CH)
PCT Filed Jan. 8, 2019, PCT No. PCT/EP2019/050335
§ 371(c)(1), (2) Date Jul. 6, 2020,
PCT Pub. No. WO2019/135008, PCT Pub. Date Jul. 11, 2019.
Claims priority of application No. 18150558 (EP), filed on Jan. 8, 2018.
Prior Publication US 2021/0063348 A1, Mar. 4, 2021
Int. Cl. G01N 27/447 (2006.01); C12N 15/10 (2006.01); C12Q 1/68 (2018.01); G01N 33/487 (2006.01)
CPC G01N 27/44756 (2013.01) [C12Q 1/68 (2013.01); G01N 33/48721 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A method for screening cells of a subject for DNA-damage comprising the steps of:
depositing a liquid sample comprising cells of the subject in at least one spot on a carrier plate, wherein the at least one spot contains a volume of 15 μL or less of the liquid sample, and wherein the at least one spot includes 75 to 400 cells of the subject to be evaluated for DNA-damage;
performing single cell gel electrophoresis on the at least one spot deposited on the carrier plate; and
determining DNA-damage for the cells of the subject present in the at least one spot based on an analysis of comets appearing in a single image taken of the at least one spot after single cell gel electrophoresis.