| CPC G01N 27/27 (2013.01) [G01N 27/4145 (2013.01); G01N 27/4146 (2013.01); G01N 29/04 (2013.01); G01N 29/2443 (2013.01); H10D 30/6734 (2025.01); H10D 30/6755 (2025.01); H10D 62/118 (2025.01); H10N 30/302 (2023.02); G01N 2291/023 (2013.01)] | 20 Claims |

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1. A biosensor system for detecting the biofilm formation and development, comprising a dual gate thin-film transistor (DGTFT) biosensor and a bulk acoustic wave (BAW) sensor, where the DGTFT biosensor comprising
(a) a DGTFT transducer, comprising
a first substrate;
a bottom biasing gate deposited on the first substrate;
a bottom dielectric layer deposited on a top surface of the bottom biasing gate;
a channel layer comprising semiconducting MgxZn1-xO deposited on a top surface of the bottom dielectric layer, wherein 0<x≤0.06;
a top gate dielectric layer deposited on the channel layer;
a top sensing gate deposited on the top gate dielectric layer; and
a drain electrode and a source electrode each electrically connected to the channel layer; and
(b) a sensing pad receptor, comprising
a second substrate;
an electrode layer comprising an array of electrode patterns with different surface areas deposited on the second substrate;
a sensing nanostructure layer deposited and patterned on the array of electrode patterns, wherein the sensing nanostructure layer comprises MgyZn1-yO, wherein 0≤y≤0.1; and
wherein the sensing pad receptor is electrically connected to the DGTFT transducer;
where the BAW sensor is a nanostructure-modified quartz crystal microbalance (QCM) or thin film bulk acoustic wave resonator (TFBAR);
wherein the nanostructure-modified QCM comprises:
a commercial quartz crystal piezoelectric layer between a first top electrode and a first bottom electrode;
a nanostructure layer comprises MgyZn1-yO, wherein y is specified as the Mg composition;
wherein the MgyZn1-yO of the nanostructure layer and the MgyZn1-yO of the sensing nanostructure layer are the same;
wherein the nanostructure-modified TFBAR comprises:
a third substrate;
a second bottom electrode deposited and patterned on the third substrate;
a piezoelectric film;
a second top electrode deposited and patterned on a top surface of the piezoelectrode film;
a MgyZn1-yO nanostructure layer deposited and patterned on the top electrode surface of the said the TFBAR, wherein y is as defined above.
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