US 12,270,749 B2
Imaging flow cytometer
Sadao Ota, Tokyo (JP); and Masashi Ugawa, Tokyo (JP)
Assigned to THE UNIVERSITY OF TOKYO, Tokyo (JP)
Appl. No. 17/924,135
Filed by The University of Tokyo, Tokyo (JP)
PCT Filed Sep. 10, 2021, PCT No. PCT/JP2021/033328
§ 371(c)(1), (2) Date Nov. 9, 2022,
PCT Pub. No. WO2022/054908, PCT Pub. Date Mar. 17, 2022.
Claims priority of application No. 2020-152331 (JP), filed on Sep. 10, 2020.
Prior Publication US 2023/0175949 A1, Jun. 8, 2023
Int. Cl. G01N 15/1434 (2024.01); G01N 15/1404 (2024.01)
CPC G01N 15/1434 (2013.01) [G01N 15/1404 (2013.01); G01N 2015/142 (2013.01); G01N 2015/1443 (2013.01)] 7 Claims
OG exemplary drawing
 
1. An imaging flow cytometer, comprising:
a flow channel in which an observation object flows and a length in a width direction is longer than a length in a height direction such that a flow rate distribution in the width direction is constant regardless of positions within the flow channel in the width direction except for the vicinity of walls of the flow channel;
an acoustic element configured to apply acoustic waves as standing waves to the flow channel;
a light source configured to irradiate the flow channel with illumination light;
an image sensor configured to image at least a line included in a cross section of the observation object crossing a flow line direction which is a direction in which the observation object flows in the flow channel by measuring or imaging the observation object passing through a position irradiated with the illumination light; and
scanned image-generating circuitry configured to generate an image in which the observation object is scanned in the flow line direction on the basis of a plurality of captured images acquired by the image sensor imaging the line in a time series, wherein
the acoustic element is configured to apply the acoustic waves as the standing waves to the flow channel such that rotation of the observation object does not occur during a process of imaging by the image sensor.