US 12,270,079 B2
Analysis method and kit for diagnosis of urolithiasis
Kwang-Hyun Baek, Seoul (KR); and Jun-Hyeok Park, Seongnam-si (KR)
Assigned to CHA UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION, Pocheon-si (KR)
Appl. No. 17/310,163
Filed by CHA UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION, Pocheon-si (KR)
PCT Filed Jan. 22, 2020, PCT No. PCT/KR2020/001113
§ 371(c)(1), (2) Date Jul. 22, 2021,
PCT Pub. No. WO2020/153759, PCT Pub. Date Jul. 30, 2020.
Claims priority of application No. 10-2019-0008510 (KR), filed on Jan. 23, 2019.
Prior Publication US 2022/0090198 A1, Mar. 24, 2022
Int. Cl. C12Q 1/686 (2018.01); C12Q 1/6883 (2018.01)
CPC C12Q 1/6883 (2013.01) [C12Q 1/686 (2013.01); C12Q 2600/158 (2013.01)] 6 Claims
OG exemplary drawing
 
1. A method for diagnosis and treatment of urolithiasis, comprising
(a) measuring an expression level of a gene encoding a YOD1, USP6, USP19, PSMD14, or USP26 protein in a biological sample from a subject to be diagnosed,
(b) comparing the expression level obtained in step (a) and a corresponding expression level in a person without urolithiasis,
(c) diagnosing the subject with urolithiasis based on a finding that either the expression level of the gene encoding the USP6 protein was significantly higher than that of a person without urolithiasis or the expression level of the gene encoding the USP19, PSMD14, USP26, or YOD1 protein was significantly lower than that of a person without urolithiasis, and
(d) providing the urolithiasis patient with a surgical treatment of extracorporeal shock wave lithotripsy or percutaneous nephrolithotomy, or a non-surgical treatment allowing a kidney stone to be discharged naturally without surgery.