US 12,270,067 B2
Nanoparticle transducer sensors and methods of use thereof
Daniel T. Chiu, Seattle, WA (US); Jiangbo Yu, Seattle, WA (US); Changfeng Wu, Changchun (CN); and Kai Sun, Changchun (CN)
Assigned to University of Washington, Seattle, WA (US); and Lamprogen, Inc., Seattle, WA (US)
Filed by University of Washington, Seattle, WA (US); and LAMPROGEN, INC., Seattle, WA (US)
Filed on Nov. 23, 2022, as Appl. No. 18/058,530.
Application 18/058,530 is a continuation of application No. 16/307,363, granted, now 11,542,539, previously published as PCT/US2017/035983, filed on Jun. 5, 2017.
Claims priority of application No. PCT/CN2016/084986 (WO), filed on Jun. 6, 2016.
Prior Publication US 2023/0077385 A1, Mar. 16, 2023
Int. Cl. G01N 21/64 (2006.01); B82Y 15/00 (2011.01); B82Y 30/00 (2011.01); C09K 11/07 (2006.01); C12Q 1/00 (2006.01)
CPC C12Q 1/006 (2013.01) [C09K 11/07 (2013.01); G01N 21/64 (2013.01); B82Y 15/00 (2013.01); B82Y 30/00 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A nanoparticle transducer for analyte concentration measurements, comprising:
a nanoparticle comprising one or more chromophores; and
an enzyme covalently coupled to the nanoparticle and configured to catalyze a reaction comprising a plurality of reaction elements;
wherein the reaction elements comprise one or more reactants including the analyte and one or more products, wherein an amount of fluorescence emitted from the one or more chromophores is determined by a concentration of a reaction element of the plurality of reaction elements, wherein the one or more chromophores comprises a chromophore sensitive to the reaction element.