	US 12,270,065 B2
	Methods for improving titer and purity of beta carotene fermentation in Blakeslea trispora (beta-carotene fermentation method)
John Royer, Kaiseraugst (CH); Emily Davis Streaker, Kaiseraugst (CH); and Warren Nelson Itterly, Kaiseraugst (CH)
Assigned to DSM IP ASSETS B.V., Heerlen (NL)
Appl. No. 17/631,647
Filed by DSM IP Assets B.V., Heerlen (NL)
PCT Filed Aug. 3, 2020, PCT No. PCT/EP2020/071767 § 371(c)(1), (2) Date Jan. 31, 2022, PCT Pub. No. WO2021/019101, PCT Pub. Date Feb. 4, 2021.
Claims priority of provisional application 62/881,789, filed on Aug. 1, 2019.
Prior Publication US 2022/0290205 A1, Sep. 15, 2022
Int. Cl. C12P 23/00 (2006.01); C12N 1/38 (2006.01); C12P 5/00 (2006.01)

CPC C12P 23/00 (2013.01) [C12N 1/38 (2013.01); C12P 5/007 (2013.01)]

12 Claims

1. A process for fermentative production of beta-carotene, the process comprising:

(a) conducting fermentation production of beta-carotene with Blakeslea trispora as a carotenoid-producing host cell, and

(b) adding during the fermentation of step (a) an amount of 0.01 to 1.0% (w/v) of curcumin effective to increase the beta-carotene during fermentation by at least 12% as compared to fermentation without addition of curcumin.