US 12,270,061 B2
Modified polypeptide of glutamine synthetase and method of producing L-glutamine using the same
Su Jin Choi, Seoul (KR); Imsang Lee, Seoul (KR); Heeyeong Kim, Seoul (KR); Byeong Soo Kim, Seoul (KR); and Kwang Woo Lee, Seoul (KR)
Assigned to CJ CHEILJEDANG CORPORATION, Seoul (KR)
Appl. No. 17/604,896
Filed by CJ CHEILJEDANG CORPORATION, Seoul (KR)
PCT Filed Mar. 4, 2021, PCT No. PCT/KR2021/002652
§ 371(c)(1), (2) Date Oct. 19, 2021,
PCT Pub. No. WO2021/177731, PCT Pub. Date Sep. 10, 2021.
Claims priority of application No. 10-2020-0027322 (KR), filed on Mar. 4, 2020.
Prior Publication US 2022/0396815 A1, Dec. 15, 2022
Int. Cl. C12P 13/14 (2006.01); C12N 9/00 (2006.01); C12N 15/10 (2006.01); C12N 15/77 (2006.01); C12R 1/15 (2006.01); C40B 40/02 (2006.01); C40B 40/10 (2006.01)
CPC C12P 13/14 (2013.01) [C12N 9/93 (2013.01); C12N 15/77 (2013.01); C40B 40/10 (2013.01); C12N 15/1034 (2013.01); C12R 2001/15 (2021.05); C40B 40/02 (2013.01)] 15 Claims
 
1. A modified polypeptide having glutamine synthetase activity, wherein the modified polypeptide has a sequence identity of 97% or more and less than 100% with the amino acid sequence of SEQ ID NO: 1, and wherein an amino acid corresponding to position 401, 402, or 404 of the amino acid sequence of SEQ ID NO: 1 is substituted with a different amino acid.