US 12,270,060 B2
Production method for L-cyclic amino acids
Masaharu Mizutani, Hyogo (JP); Ryoma Miyake, Tokyo (JP); and Hiroshi Kawabata, Tokyo (JP)
Assigned to UBE CORPORATION, Yamaguchi (JP)
Appl. No. 17/606,123
Filed by UBE Corporation, Yamaguchi (JP)
PCT Filed May 15, 2020, PCT No. PCT/JP2020/019517
§ 371(c)(1), (2) Date Oct. 25, 2021,
PCT Pub. No. WO2020/218624, PCT Pub. Date Oct. 29, 2020.
Claims priority of application No. 2019-084234 (JP), filed on Apr. 25, 2019.
Prior Publication US 2022/0275410 A1, Sep. 1, 2022
Int. Cl. C12P 13/04 (2006.01); C12N 9/10 (2006.01); C12N 15/70 (2006.01); C12P 41/00 (2006.01)
CPC C12P 13/04 (2013.01) [C12N 9/1096 (2013.01); C12N 15/70 (2013.01); C12P 41/006 (2013.01)] 7 Claims
 
1. A method of producing an L-cyclic amino acid, the method comprising bringing a cyclic amino acid having a double bond at the 1-position and having the following general formula (I):

OG Complex Work Unit Chemistry
wherein A is an alkylene chain which has a chain length of from 1 to 4 atoms, which contains or does not contain at least one hetero atom selected from the group consisting of a sulfur atom, an oxygen atom and a nitrogen atom, in the chain or at the end of the chain, and which has or does not have a substituent,
into contact with a polypeptide shown in (A), (B) or (C) below, a microorganism or cell having the ability to produce said polypeptide or comprising said polypeptide, a processed product of the microorganism or the cell, and/or a culture liquid obtained by culturing the microorganism or the cell and comprising said polypeptide, to produce an L-cyclic amino acid having the following general formula (II):

OG Complex Work Unit Chemistry
wherein A is the same as defined above:
(A) a polypeptide comprising the amino acid sequence of SEQ ID NO: 2, 4, 6, 8, 10 or 12;
(B) a polypeptide which comprises the amino acid sequence of SEQ ID NO: 2, 4, 6, 8, 10 or 12 except that 1 to 10 amino acids are deleted, conservatively substituted and/or added, and which has the ability to catalyze the reaction of the following formula (1) to produce the L-cyclic amino acid:

OG Complex Work Unit Chemistry
wherein A is the same as defined above;
or
(C) a polypeptide which comprises an amino acid sequence having a sequence identity of 95% or more to the amino acid sequence of SEQ ID NO: 2, 4, 6, 8, 10 or 12, and which has the ability to catalyze the reaction of the formula (1) to produce the L-cyclic amino acid.