US 12,270,048 B2
Manufacturing method for substrate on which nerve cells are arranged
Hidekazu Yaginuma, Kanagawa (JP); Masayuki Yumoto, Kanagawa (JP); Tatsuya Sameshima, Kanagawa (JP); Naoki Satoh, Kanagawa (JP); Natsuko Iwashita, Tokyo (JP); Takahiko Matsumoto, Kanagawa (JP); Yusuke Nonoyama, Kanagawa (JP); and Takehiro Yamazaki, Saitama (JP)
Assigned to Ricoh Company, Ltd., Tokyo (JP)
Filed by RICOH COMPANY, LTD., Tokyo (JP)
Filed on Mar. 2, 2021, as Appl. No. 17/189,434.
Claims priority of application No. 2020-050906 (JP), filed on Mar. 23, 2020; application No. 2020-115635 (JP), filed on Jul. 3, 2020; and application No. 2020-217760 (JP), filed on Dec. 25, 2020.
Prior Publication US 2021/0292709 A1, Sep. 23, 2021
Int. Cl. C12N 5/079 (2010.01)
CPC C12N 5/0618 (2013.01) [C12N 2500/70 (2013.01); C12N 2537/00 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A manufacturing method, the method comprising:
a step of forming one or a plurality of liquid pools by ejecting a plurality of liquid droplets comprising nerve cells from an inkjet head onto a substrate, to form one or a plurality of liquid pools, wherein the substrate has a region in which a cell adhesive material is arranged and a region in which a cell non-adhesive material is arranged; and
a step of incubating the liquid pool until the nerve cells in the liquid pool sediment and temporarily adhere onto the substrate to form a cell aggregate,
wherein the diameter per one liquid pool is 400 μm or less, and
the density of nerve cells per one liquid pool is 105 cells/cm2 or more.