US 12,270,045 B2
Method for preparing an irradiated platelet lysate
Bruno Delorme, Marcq-en-Baroeul (FR); and Sabrina Viau, Wasquehal (FR)
Assigned to Maco Pharma, Mouvaux (FR)
Appl. No. 16/969,600
Filed by MACO PHARMA, Mouvaux (FR)
PCT Filed Feb. 12, 2019, PCT No. PCT/EP2019/053472
§ 371(c)(1), (2) Date Aug. 13, 2020,
PCT Pub. No. WO2019/158540, PCT Pub. Date Aug. 22, 2019.
Claims priority of application No. 1851307 (FR), filed on Feb. 15, 2018.
Prior Publication US 2021/0032590 A1, Feb. 4, 2021
Int. Cl. C12N 5/00 (2006.01); A61K 41/17 (2020.01); C12N 5/078 (2010.01); C12N 9/64 (2006.01)
CPC C12N 5/0018 (2013.01) [A61K 41/17 (2020.01); C12N 5/0644 (2013.01); C12N 9/6437 (2013.01); C12N 9/644 (2013.01); C12N 9/6443 (2013.01); C12N 2529/10 (2013.01)] 19 Claims
 
1. A method for preparing an irradiated platelet lysate comprising the following steps:
providing a platelet lysate to obtain a starting platelet lysate, the starting platelet lysate comprising platelet factors including growth factors and further comprising plasma proteins including a plurality of coagulation factors and proteins other than the coagulation factors;
subjecting the starting platelet lysate to double irradiation with UVC radiation having a wavelength between 200 and 280 nm and with ionizing radiation having a wavelength less than or equal to 100 nm to obtain a platelet lysate irradiated with UVC radiation and with ionizing radiation,
the double irradiation with UVC radiation and with ionizing radiation being arranged to retain at least 75% of the total protein concentration of the starting platelet lysate while reducing by at least 40% the concentration of at least one of the coagulation factors selected from the group consisting of fibrinogen, factor II, factor VII, factor IX, factor X, and factor XI of the starting platelet lysate.