US 12,270,039 B2
Compositions and methods to increase oleic acid content in soybeans
Khalid Meksem, Carbondale, IL (US); Naoufal Lakhssassi, Carbondale, IL (US); and Dounya Knizia, Carbondale, IL (US)
Assigned to Board of Trustees of Southern Illinois University, Carbondale, IL (US)
Filed by Board of Trustees of Southern Illinois University, Carbondale, IL (US)
Filed on May 16, 2022, as Appl. No. 17/745,148.
Claims priority of provisional application 63/188,993, filed on May 14, 2021.
Prior Publication US 2022/0364106 A1, Nov. 17, 2022
Int. Cl. C12N 15/82 (2006.01)
CPC C12N 15/8247 (2013.01) 5 Claims
 
1. A transgenic soybean plant with increased oleic acid content transformed with a polynucleotide encoding a fatty acid desaturase (FAD) related promoter that functions in the soybean plant operably linked to a polynucleotide encoding a polypeptide having FAD activity,
wherein the polypeptide having FAD activity comprises a sequence at least 95% identical to wild type “Forrest” FAD2-2E amino acid sequence (SEQ ID NO: 29) and further comprises one or more mutations selected from the group consisting of A21T, S56F, S56T, G110E, G110R, P112S, A117V, L133F, P168S, W199R, G202E, R209K, D210N, H236Y, E241K, G251R, E252K, E268V, and P277S, and
wherein the FAD related promoter comprises a sequence selected from the group consisting of wild type “Forrest” FAD2-2A (SEQ ID NO: 15), wild type “Forrest” FAD2-2B (SEQ ID NO: 18), wild type “Forrest” FAD2-2C (SEQ ID NO: 21), wild type “Forrest” FAD2-2D (SEQ ID NO: 24), and wild type “Forrest” FAD2-2E (SEQ ID NO: 27) nucleotide sequence.