US 12,270,030 B2
Method for stabilizing nucleic acid nanostructures
Thomas Gerling, Munich (DE); and Hendrik Dietz, Haar (DE)
Assigned to Technische Universität München, Munich (DE)
Appl. No. 15/734,938
Filed by Technische Universitat Munchen, Munich (DE)
PCT Filed Jun. 5, 2019, PCT No. PCT/EP2019/064707
§ 371(c)(1), (2) Date Dec. 3, 2020,
PCT Pub. No. WO2019/234122, PCT Pub. Date Dec. 12, 2019.
Claims priority of application No. 102018004454.9 (DE), filed on Jun. 5, 2018.
Prior Publication US 2021/0230587 A1, Jul. 29, 2021
Int. Cl. C12Q 1/68 (2018.01); C07H 21/04 (2006.01); C12N 15/113 (2010.01); B82Y 40/00 (2011.01)
CPC C12N 15/113 (2013.01) [C07H 21/04 (2013.01); B82Y 40/00 (2013.01); C12N 2320/52 (2013.01)] 22 Claims
 
1. A method for increasing the stability of a non-naturally occurring nucleic acid nanostructure, wherein said nanostructure comprises at least one double-helical substructure comprising a first single-stranded polynucleotide having a sequence binding via the formation of hydrogen bonds to at least two non-contiguous sequence stretches present on one or more complementary second single-stranded polynucleotides, the method comprising: exposing said nucleic acid nanostructure to UV irradiation, wherein said step of exposing said nucleic acid nanostructure to UV irradiation results in the formation of at least one chemical bond between two pyrimidine nucleotides, wherein each of the two pyrimidine nucleotides is independently selected from thymidine and cytosine.