US 12,270,024 B2
Engineered microorganisms expressing acetoacetyl-COA reductase variants and methods for increasing proportion of 3-hydroxyhexanoic acid in PHA
Jin Yin, Guangdong (CN); Yu Wang, Guangdong (CN); Jiajia Li, Guangdong (CN); Jie Hou, Guangdong (CN); Liang Zou, Guangdong (CN); and Zixian Chen, Guangdong (CN)
Assigned to SHENZHEN BLUEPHA BIOSCIENCES CO., LTD., Guangdong (CN)
Appl. No. 18/031,556
Filed by SHENZHEN BLUEPHA BIOSCIENCES CO., LTD., Guangdong (CN)
PCT Filed Jun. 28, 2022, PCT No. PCT/CN2022/101796
§ 371(c)(1), (2) Date Apr. 12, 2023,
PCT Pub. No. WO2023/193352, PCT Pub. Date Oct. 12, 2023.
Claims priority of application No. 202210353444.8 (CN), filed on Apr. 6, 2022.
Prior Publication US 2024/0263130 A1, Aug. 8, 2024
Int. Cl. C12N 1/20 (2006.01); C12N 9/04 (2006.01); C12N 9/10 (2006.01); C12N 9/88 (2006.01); C12N 15/74 (2006.01); C12N 15/90 (2006.01); C12P 7/625 (2022.01); C12R 1/01 (2006.01)
CPC C12N 1/20 (2013.01) [C12N 9/0006 (2013.01); C12N 9/1029 (2013.01); C12N 9/88 (2013.01); C12N 15/74 (2013.01); C12N 15/90 (2013.01); C12P 7/625 (2013.01); C12N 2510/00 (2013.01); C12R 2001/01 (2021.05); C12Y 101/01036 (2013.01); C12Y 402/01119 (2013.01)] 12 Claims
OG exemplary drawing
 
1. An engineered Ralstonia eutropha strain, wherein the engineered Ralstonia eutropha strain expresses an acetoacetyl-CoA reductase variant, the acetoacetyl-CoA reductase variant having the amino acid sequence of SEQ ID NO. 10.