US 12,270,021 B2
Device and method for microorganism cell disruption by extrusion
Marcio Arêdes Martins, Viçosa (BR); Dilson Novais Rocha, Viçosa (BR); Mauricio De Oliveira Leite, Viçosa (BR); Bruno Bezerra Vieira, Viçosa (BR); Rafael Richard João, Rio de Janeiro (BR); and Leonardo Brantes Bacellar Mendes, Rio de Janeiro (BR)
Assigned to Petróleo Brasileiro S.A.—PETROBRAS, Rio de Janeiro (BR); and Universidade Federal De Viçosa, Florianópolis (BR)
Appl. No. 16/980,322
Filed by PETRÓLEO BRASILEIRO S.A.—PETROBRAS, Rio de Janeiro (BR); and UNIVERSIDADE FEDERAL DE VIÇOSA, Viçosa (BR)
PCT Filed Mar. 8, 2019, PCT No. PCT/BR2019/050074
§ 371(c)(1), (2) Date Sep. 11, 2020,
PCT Pub. No. WO2019/173888, PCT Pub. Date Sep. 19, 2019.
Claims priority of application No. 10 2018 004973 9 (BR), filed on Mar. 13, 2018.
Prior Publication US 2021/0115388 A1, Apr. 22, 2021
Int. Cl. C12M 1/00 (2006.01); C12M 1/02 (2006.01); C12M 1/26 (2006.01); C12M 1/34 (2006.01); C12M 1/36 (2006.01); C12N 1/06 (2006.01)
CPC C12M 47/06 (2013.01) [C12M 33/00 (2013.01); C12M 41/18 (2013.01); C12M 41/40 (2013.01); C12M 41/48 (2013.01); C12N 1/066 (2013.01)] 13 Claims
OG exemplary drawing
 
1. A device for cell disruption of microorganisms by extrusion, characterized in that the device comprises:
an inlet duct for a microorganism suspension;
an annular channel downstream of the inlet duct and in communication therewith, adapted for disruption of microorganism cells, the annular channel being formed by an external part and an internal part, the internal part being positioned inside a cavity formed by the external part;
a pump adapted to pump a suspension of microorganisms through the annular channel; and
an outlet duct downstream of the annular channel and in communication therewith, for output of ruptured cells,
wherein
the internal part is adjustable with respect to the inner cavity formed by the external part by an adjustment mechanism to regulate the diameter of the annular channel.