US 12,270,019 B2
Automated method
Niraj Kumar Doshi, Valencia, CA (US); Jugal Kapadia, Valencia, CA (US); Matthew Krywcun, Laguna Niguel, CA (US); David Allen Fencil, Valencia, CA (US); David Melbye, Valencia, CA (US); Katie Bush, Marblehead, MA (US); Carl Swindle, San Clemente, CA (US); Paul Faucher, San Diego, CA (US); Matthew Richard Johnson, San Diego, CA (US); Adam Livingston, San Diego, CA (US); Michael MacCollum, San Diego, CA (US); Evan Misuraca, San Diego, CA (US); Kyle Stewart, San Diego, CA (US); Mikhail Tikh, San Diego, CA (US); and Austin Wolf, San Diego, CA (US)
Assigned to AVITA Medical Americas, LLC, Valencia, CA (US)
Filed by AVITA Medical Americas, LLC, Valencia, CA (US)
Filed on Dec. 22, 2023, as Appl. No. 18/395,405.
Prior Publication US 2024/0207841 A1, Jun. 27, 2024
Int. Cl. C12M 1/00 (2006.01); B01L 3/00 (2006.01); C12M 1/02 (2006.01); C12M 1/12 (2006.01); C12M 1/26 (2006.01); C12M 1/32 (2006.01); C12M 1/36 (2006.01); C12M 3/00 (2006.01); C12M 3/02 (2006.01); C12M 3/04 (2006.01); C12M 3/08 (2006.01); G01N 1/28 (2006.01)
CPC C12M 41/48 (2013.01) [B01L 3/50255 (2013.01); C12M 3/02 (2013.01); C12M 3/08 (2013.01); C12M 21/08 (2013.01); C12M 23/12 (2013.01); C12M 23/38 (2013.01); C12M 23/42 (2013.01); C12M 23/50 (2013.01); C12M 25/04 (2013.01); C12M 27/10 (2013.01); C12M 33/00 (2013.01); C12M 33/14 (2013.01); C12M 41/18 (2013.01); G01N 1/286 (2013.01); B01L 2300/042 (2013.01); G01N 2001/2866 (2013.01)] 24 Claims
OG exemplary drawing
 
1. A method for automated preparation of a regenerative epidermal suspension, comprising:
receiving, by a processor, an initiation signal, wherein the initiation signal indicates that a cartridge has been placed on a sensor, wherein the cartridge comprises:
a cover,
a cup containing a tissue sample, and
a well plate situated beneath the cover, the well plate comprising a first well containing a quantity of enzyme solution and a second well containing a quantity of buffer solution;
via the processor, executing a sequence, wherein the sequence comprises:
actuating a tissue disaggregator against the tissue sample in the presence of the enzyme solution with the cup positioned in the first well;
raising the cup to an upper position;
rotating the well plate to position the second well beneath the cup;
lowering the cup to a lower position within the second well; and
actuating the tissue disaggregator against the tissue sample in the presence of the buffer solution with the cup positioned in the second well.