| CPC C07K 16/2809 (2013.01) [A61K 39/461 (2023.05); A61K 39/4632 (2023.05); A61K 39/464486 (2023.05); A61P 35/00 (2018.01); C07K 14/7051 (2013.01); C07K 16/30 (2013.01); C07K 16/468 (2013.01); C07K 2317/24 (2013.01); C07K 2317/32 (2013.01); C07K 2317/55 (2013.01); C07K 2317/565 (2013.01); C07K 2317/567 (2013.01); C07K 2317/622 (2013.01); C07K 2317/92 (2013.01)] | 8 Claims |
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1. A protein that binds both (a) a complex of MAGE-A and human major histocompatibility complex (MHC) protein and (b) a TCRαß, wherein said protein comprises
(a) a first polypeptide comprising
an amino acid sequence that is at least 98% identical to SEQ ID NO: 136 comprising (1) a beta variable (Vβ) domain comprising
(i) a CDR1 comprising the amino acid sequence of SEQ ID NO: 62,
(ii) a CDR2 comprising the amino acid sequence of SEQ ID NO: 65, and
(iii) a CDR3 comprising the amino acid sequence of SEQ ID NO: 71; and
(2) an antibody light chain variable (VL) domain comprising the amino acid sequence of SEQ ID NO: 159; and
(b) a second polypeptide comprising
an amino acid sequence that is at least 98% identical to SEQ ID NO: 137 comprising (1) an alpha variable (Vα) domain comprising
(i) a CDR1 comprising the amino acid sequence of SEQ ID NO: 5,
(ii) a CDR2 comprising the amino acid sequence of SEQ ID NO: 56, and
(iii) a CDR3 comprising the amino acid sequence of SEQ ID NO: 35, and
(2) an antibody heavy chain variable (VH) domain comprising the amino acid sequence of SEQ ID NO: 160; and wherein
the Vα and the Vβ form a binding site that binds to the MAGE-A/MHC complex and the VL and VH form a binding site that binds to the TCRαß.
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