US 12,269,869 B2
Chimeric antigen receptor effector cell switches with humanized targeting moieties and/or optimized chimeric antigen receptor interacting domains and uses thereof
Travis S. Young, La Jolla, CA (US); Leonard Presta, La Jolla, CA (US); David Rodgers, La Jolla, CA (US); Eric Hampton, La Jolla, CA (US); Timothy Wright, La Jolla, CA (US); Peter G. Schultz, La Jolla, CA (US); Eduardo Laborda, La Jolla, CA (US); Elvira Khialeeva, La Jolla, CA (US); and Sophie Viaud, La Jolla, CA (US)
Assigned to The Scripps Research Institute, La Jolla, CA (US)
Filed by The Scripps Research Institute, La Jolla, CA (US)
Filed on Aug. 30, 2021, as Appl. No. 17/461,365.
Application 17/461,365 is a continuation of application No. 16/343,353, granted, now 11,174,306, previously published as PCT/US2017/057460, filed on Oct. 19, 2017.
Claims priority of provisional application 62/410,315, filed on Oct. 19, 2016.
Prior Publication US 2022/0073597 A1, Mar. 10, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 16/18 (2006.01); A61P 35/00 (2006.01); C07K 14/705 (2006.01); C07K 14/725 (2006.01); C07K 16/28 (2006.01); A61K 39/00 (2006.01)
CPC C07K 16/18 (2013.01) [A61P 35/00 (2018.01); C07K 14/7051 (2013.01); C07K 14/70517 (2013.01); C07K 14/70521 (2013.01); C07K 14/70578 (2013.01); C07K 16/2803 (2013.01); A61K 2039/507 (2013.01); C07K 2317/24 (2013.01); C07K 2317/34 (2013.01); C07K 2317/55 (2013.01); C07K 2317/565 (2013.01); C07K 2317/622 (2013.01); C07K 2317/73 (2013.01); C07K 2317/76 (2013.01); C07K 2317/92 (2013.01); C07K 2319/02 (2013.01); C07K 2319/03 (2013.01); C07K 2319/30 (2013.01); C07K 2319/33 (2013.01)] 24 Claims
 
1. A chimeric antigen receptor-effector cell (CAR-EC) expressing a humanized chimeric antigen receptor (CAR), wherein the humanized CAR comprises an extracellular domain, a transmembrane domain, and an intracellular signaling domain; wherein the extracellular domain comprises a humanized anti-GCN4 scFv comprising light chain CDRs 1-3 and heavy chain CDRs 1-3, respectively, set forth as GSSTGAVTTSNYAS (SEQ ID NO: 425), GTNNRAP (SEQ ID NO:426), VLWYSDHWV (SEQ ID NO:427), GFLLTDYGVN (SEQ ID NO:428), VIWGDGITD (SEQ ID NO:429), and GLFDY (SEQ ID NO:430).