US 12,269,852 B2
Processes for producing a fermentation product
Marc Dominique Morant, Frederiksberg (DK); Tomoko Matsui, Chiba (JP); Kirk Matthew Schnorr, Holte (DK); Shiro Fukuyama, Chiba (JP); Noriko Tsutsumi, Chiba (JP); and Zhengfang Kang, Raleigh, NC (US)
Assigned to NOVOZYMES A/S, Bagsvaerd (DK)
Filed by NOVOZYMES A/S, Bagsvaerd (DK)
Filed on Sep. 28, 2022, as Appl. No. 17/936,076.
Application 17/936,076 is a division of application No. 16/624,164, granted, now 11,584,783, previously published as PCT/US2018/039443, filed on Jun. 26, 2018.
Claims priority of provisional application 62/526,133, filed on Jun. 28, 2017.
Prior Publication US 2024/0101991 A1, Mar. 28, 2024
Int. Cl. C12P 7/06 (2006.01); C07K 14/39 (2006.01); C07K 14/395 (2006.01); C12N 9/24 (2006.01); C12N 9/26 (2006.01); C12N 15/81 (2006.01); C12P 19/14 (2006.01)
CPC C07K 14/39 (2013.01) [C12N 9/2402 (2013.01); C12N 9/2408 (2013.01); C12N 15/81 (2013.01); C12P 7/06 (2013.01); C12P 19/14 (2013.01); C12Y 302/01001 (2013.01); C12Y 302/01003 (2013.01); C12Y 302/01028 (2013.01); C12Y 302/01093 (2013.01)] 12 Claims
 
1. A process of producing ethanol, comprising
(a) liquefying a starch-containing material with an alpha-amylase to form a liquefied material;
(b) saccharifying the liquefied material;
(c) fermenting using a fermentation organism;
wherein a glucoamylase and a polypeptide having trehalase activity and at least 75% sequence identity to the mature polypeptide of SEQ ID NO: 23 are present and/or added during saccharification step (b) or fermentation step (c) and wherein sequence identity is determined using the Needleman-Wunsch algorithm as implemented in the Needle program of the EMBOSS package, version 5.0.0, using a gap open penalty of 10, a gap extension penalty of 0.5, and EBLOSUM62 substitution matrix.