US 12,269,851 B2
Polypeptide and method of producing IMP using the same
Jin Ah Rho, Suwon-si (KR); Byoung Hoon Yoon, Seoul (KR); So-Jung Park, Suwon-si (KR); Min Ji Baek, Suwon-si (KR); and Ji Hye Lee, Anyang-si (KR)
Assigned to CJ CHEILJEDANG CORPORATION, Seoul (KR)
Filed by CJ CHEILJEDANG CORPORATION, Seoul (KR)
Filed on Nov. 29, 2022, as Appl. No. 18/059,581.
Application 18/059,581 is a division of application No. 17/833,539, filed on Jun. 6, 2022, granted, now 11,746,130.
Application 17/833,539 is a division of application No. 16/346,725, granted, now 11,384,124, issued on Jul. 12, 2022, previously published as PCT/KR2019/000168, filed on Jan. 4, 2019.
Claims priority of application No. 10-2018-0001399 (KR), filed on Jan. 4, 2018.
Prior Publication US 2023/0192780 A1, Jun. 22, 2023
Int. Cl. C07K 14/34 (2006.01); C12N 1/20 (2006.01); C12P 19/32 (2006.01); C12R 1/15 (2006.01)
CPC C07K 14/34 (2013.01) [C12N 1/20 (2013.01); C12N 1/205 (2021.05); C12P 19/32 (2013.01); C12R 2001/15 (2021.05)] 4 Claims
 
1. A method for preparing 5′-inosine monophosphate, comprising culturing a microorganism of the genus Corynebacterium comprising a protein variant having activity of exporting 5′-inosine monophosphate or a polynucleotide encoding the protein variant in a medium; and recovering 5′-inosine monophosphate from the microorganism or medium,
wherein the protein variant has at least 90% sequence identity to SEQ ID NO:2, and comprises one or more substitutions selected from the group consisting of: a substitution of the 123rd amino acid with cysteine, a substitution of the 243rd amino acid with valine, a substitution of the 387th amino acid with threonine, a substitution of the 405th amino acid with tyrosine, a substitution of the 413th amino acid with threonine, and a substitution of the 458th amino acid with lysine, or a combination thereof, in the amino acid sequence of SEQ ID NO: 2.