US 12,269,036 B2
Devices, systems, and methods for increasing droplet formation efficiency
Ivan Akhremichev, San Leandro, CA (US); Rajiv Bharadwaj, Pleasanton, CA (US); Lynna Chen, Oakland, CA (US); Mohammad Rahimi Lenji, Livermore, CA (US); Alireza Salmanzadeh, San Francisco, CA (US); Martin Sauzade, Pleasanton, CA (US); Tobias Daniel Wheeler, Pleasanton, CA (US); and Yiran Zhang, Castro Valley, CA (US)
Assigned to 10x Genomics, Inc., Pleasanton, CA (US)
Filed by 10x Genomics, Inc., Pleasanton, CA (US)
Filed on Aug. 27, 2021, as Appl. No. 17/458,757.
Application 17/458,757 is a continuation of application No. PCT/US2020/020486, filed on Feb. 28, 2020.
Claims priority of provisional application 62/960,955, filed on Jan. 14, 2020.
Claims priority of provisional application 62/872,196, filed on Jul. 9, 2019.
Claims priority of provisional application 62/811,823, filed on Feb. 28, 2019.
Claims priority of provisional application 62/811,992, filed on Feb. 28, 2019.
Prior Publication US 2021/0387195 A1, Dec. 16, 2021
Int. Cl. B01L 3/00 (2006.01); B01F 23/00 (2022.01); B01F 23/41 (2022.01); B01F 25/314 (2022.01); B01F 33/3011 (2022.01); B01F 101/23 (2022.01); B23Q 17/24 (2006.01); C07K 14/705 (2006.01); C12M 1/34 (2006.01); C12Q 1/04 (2006.01); C12Q 1/18 (2006.01); C12Q 1/686 (2018.01); G01N 15/1492 (2024.01); G01N 21/3577 (2014.01); G01N 21/359 (2014.01); G01N 21/39 (2006.01); G01N 21/45 (2006.01); G01N 21/64 (2006.01); G01N 27/414 (2006.01); G01N 30/12 (2006.01); G01N 30/68 (2006.01); G01N 30/70 (2006.01); G01N 30/72 (2006.01); G01N 30/88 (2006.01); G01N 33/00 (2006.01); G01N 33/18 (2006.01); G01N 33/50 (2006.01); G01N 33/53 (2006.01); G01N 33/543 (2006.01); G01N 33/68 (2006.01); G01N 33/74 (2006.01); H10K 10/46 (2023.01); H10K 85/00 (2023.01); H10K 85/20 (2023.01); G01N 15/10 (2006.01); G01N 15/14 (2006.01); G01N 15/149 (2024.01)
CPC B01L 3/502784 (2013.01) [B01F 23/41 (2022.01); B01F 25/31423 (2022.01); B01F 33/3011 (2022.01); G01N 15/1492 (2024.01); B01F 23/4144 (2022.01); B01F 2215/0422 (2013.01); B01L 2200/0673 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0858 (2013.01); B01L 2400/0487 (2013.01); G01N 2015/1006 (2013.01); G01N 15/1459 (2013.01); G01N 2015/1481 (2013.01); G01N 15/149 (2024.01)] 20 Claims
OG exemplary drawing
 
1. A device for producing droplets of a first liquid in a second liquid, the device comprising:
a) a sample inlet;
b) first, second, and third sample channels, each of which is in fluid communication with the sample inlet;
c) first and second reagent inlets;
d) first, second, and third reagent channels, wherein the first and second reagent channels are in fluid communication with the first reagent inlet; the third reagent channel is in fluid communication with the second reagent inlet; the first sample channel and the first reagent channel intersect at a first intersection, the second sample channel and the second reagent channel intersect at a second intersection, and the third sample channel and the third reagent channel intersect at a third intersection;
e) first, second, and third droplet formation regions; and
f) first and second collection reservoirs,
wherein the first droplet formation region is fluidically disposed between the first intersection and the first collection reservoir, the second droplet formation region is fluidically disposed between the second intersection and the first collection reservoir, and the third droplet formation region is fluidically disposed between the third intersection and the second collection reservoir.