US 12,269,035 B2
Method of determining the transfection status of a plurality of cells
Dmitry Kashanin, Dublin (IE); Vivienne Williams, Dublin (IE); and Dhruv Gandhi, County Dublin (IE)
Assigned to CELLIX LIMITED, Dublin (IE)
Filed by CELLIX LIMITED, Dublin (IE)
Filed on Feb. 26, 2021, as Appl. No. 17/186,772.
Claims priority of application No. 20159936 (EP), filed on Feb. 27, 2020.
Prior Publication US 2021/0291186 A1, Sep. 23, 2021
Int. Cl. B01L 3/00 (2006.01); G01N 27/04 (2006.01)
CPC B01L 3/502784 (2013.01) [G01N 27/041 (2013.01); B01L 2300/0645 (2013.01); B01L 2300/087 (2013.01)] 6 Claims
OG exemplary drawing
 
1. A system to determine a transfection efficiency of a cell membrane disruption treatment on at least one cell in a population of cells and monitor recovery of the at least one cell following the cell membrane disruption treatment, comprising:
a cell membrane disruption module configured to treat the population of cells to disrupt cell membranes of the population of cells;
a fluidic device comprising a microfluidic channel fluidically coupled to the cell membrane rupture module to receive treated cells from the cell membrane disruption module and a detection zone downstream of the cell membrane disruption module comprising a detection electrode module comprising two electrodes configured to detect changes in electrical impedance between the two electrodes transversely across the microfluidic channel in the detection zone as the treated cells pass the detection zone;
a pump fluidically coupled to the fluidic device and configured to pump the population of cells in a carrier liquid along the microfluidic channel at a linear velocity of 0.1 m/s to 10 m/s; and
a processor operatively coupled to the detection electrode module and configured to (a) detect a change in electrical impedance corresponding to the at least one cell passing the detection zone at a first time point immediately after the cell membrane disruption treatment, compare the change in electrical impedance with a reference change in electrical impedance corresponding to a reference cell of known transfection status, and calculate the transfection efficiency of the cell membrane disruption treatment on the least one cell based on the comparing, and (b) detect a change in electrical impedance corresponding to the at least one cell passing the detection zone at a plurality of time points after the first time point to monitor the recovery of the cell following the cell membrane disruption treatment.