| CPC B01L 3/502753 (2013.01) [B01L 3/50273 (2013.01); B01L 2200/0621 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0816 (2013.01); B01L 2400/0481 (2013.01); B01L 2400/0633 (2013.01)] | 26 Claims |

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1. A microfluidic device with a fluidic channel, comprising:
a filter positioned within a sealable portion of the fluidic channel,
a valve configured to releasably seal the sealable portion of the fluidic channel which contains the filter therein, and
a displacement pump in the sealable portion of the fluidic channel, the displacement pump being configured to displace a volume greater than a volume of the sealable portion and configured to reduce a pressure in said sealable portion of the fluidic channel which contains the filter therein, wherein the displacement pump is configured to substantially simultaneously draw fluid through or over the filter, and is configured to reduce the pressure in said sealable portion of the fluidic channel containing the filter;
wherein the filter divides the sealable portion into a first volume between said valve and the filter and a second volume between the displacement pump and the valve, wherein the first volume is smaller than the second volume, and
wherein a volume of the fluidic channel downstream of the valve is larger than the volume of the sealable portion.
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