US 12,269,003 B2
Extracellular vesicle separation method, colloidal particle and preparation method thereof
Yu-Fang Yen, Hsinchu (TW); Chien-An Chen, New Taipei (TW); and Cheng-Tai Chen, Taoyuan (TW)
Assigned to Industrial Technology Research Institute, Hsinchu (TW)
Filed by Industrial Technology Research Institute, Hsinchu (TW)
Filed on Dec. 28, 2020, as Appl. No. 17/134,496.
Claims priority of provisional application 62/954,670, filed on Dec. 30, 2019.
Prior Publication US 2021/0197162 A1, Jul. 1, 2021
Int. Cl. C08B 37/00 (2006.01); B01D 15/20 (2006.01); B01D 15/38 (2006.01); B01D 15/42 (2006.01); B01J 13/04 (2006.01); B01J 13/18 (2006.01); B01J 13/20 (2006.01)
CPC B01J 13/185 (2013.01) [B01D 15/206 (2013.01); B01D 15/38 (2013.01); B01D 15/426 (2013.01); B01J 13/04 (2013.01); B01J 13/20 (2013.01); C08B 37/0039 (2013.01); B01D 2221/10 (2013.01)] 5 Claims
 
1. A preparation method of a colloidal particle, the colloidal particle being used for extracellular vesicle separation, and the preparation method comprising:
preparing an agarose solution with a concentration of 2 wt % to 6 wt % with water and agarose and heating the agarose solution to 90° C. to 100° C., and adding a surfactant and a mineral oil into the agarose solution for emulsification to form a first colloidal material;
sieving the first colloidal material with a sieve to obtain a second colloidal material with a particle size ranging from 25 μm to 500 μm, and washing the second colloidal material with a detergent to remove oil stains and impurities;
adding a cross-linking agent with a concentration of 0.1 M to 0.5 M into the washed second colloidal material to form a third colloidal material through stirring; and
washing the third colloidal material with an organic solvent, and then surface-modifying the third colloidal material with a modification solution comprising biocompatible molecules to obtain a colloidal particle surface-modified with the biocompatible molecules,
wherein the biocompatible molecules comprise sodium carboxymethyl cellulose (CMC), methyl cellulose (MC), glycine, aspartic acid, glutamic acid, bovine serum albumin (BSA), fetal bovine serum (FBS), or a combination thereof,
wherein the detergent comprises sodium dodecyl sulfate (SDS), sodium deoxycholate (DOC), cetyltrimethylammonium bromide (CTAB), or a combination thereof.