US 12,269,000 B2
Colloidal particles functionalized homogeneously by biomolecules
Jacques Fattaccioli, Paris (FR); Lea Pinon, Vincennes (FR); and Lorraine Montel, Cachen (FR)
Assigned to Paris Sciences et Lettres, Paris (FR); Centre National de la Recherche Scientifique (CNRS), Paris (FR); and Sorbonne Universite, Paris (FR)
Appl. No. 17/058,277
Filed by Paris Sciences et Lettres, Paris (FR); Centre National de la Recherche Scientifique (CNRS), Paris (FR); and Sorbonne Universite, Paris (FR)
PCT Filed May 24, 2019, PCT No. PCT/EP2019/063521
§ 371(c)(1), (2) Date Nov. 24, 2020,
PCT Pub. No. WO2019/224375, PCT Pub. Date Nov. 28, 2019.
Claims priority of application No. 1854366 (FR), filed on May 24, 2018.
Prior Publication US 2021/0213410 A1, Jul. 15, 2021
Int. Cl. B01J 13/00 (2006.01); C09K 23/00 (2022.01); C12Q 1/02 (2006.01); G01N 15/10 (2024.01)
CPC B01J 13/0039 (2013.01) [B01J 13/0047 (2013.01); C09K 23/00 (2022.01); C12Q 1/02 (2013.01); G01N 15/1012 (2013.01); G01N 2015/1014 (2024.01)] 15 Claims
 
1. A method for obtaining a colloid comprising functionalized liquid colloidal particles comprising the following steps:
a) dispersing an oil in an aqueous solution comprising a fragmentation surfactant, leading to obtaining an emulsion comprising oil droplets suspended in an aqueous phase;
b) dissolving lipids aimed at functionalizing the oil droplets in a polar aprotic solvent, leading to obtaining a functionalization solution;
c) preparing a functionalization mixture comprising the emulsion and the functionalization solution, the volume fraction of the polar aprotic solvent in the functionalization mixture being comprised between 1 and 15%;
d) incubating the functionalization mixture, during which at least a part of the lipids initially present in the functionalization solution are adsorbed on the surface of the oil droplets initially present in the emulsion; and
e) eliminating non-adsorbed lipids during step d);
thus allowing to obtain a colloid comprising functionalized liquid colloidal particles consisting of the oil droplets obtained after step a) on the surface of which the lipids are adsorbed during step d).