US 12,268,782 B2
Preparation method of fatty acid liposome for VC encapsulation
Yongmei Xia, Wuxi (CN); Huan Liu, Wuxi (CN); Xinyu Meng, Wuxi (CN); Yun Fang, Wuxi (CN); Ye Fan, Wuxi (CN); Jie Shen, Wuxi (CN); and Xiang Liu, Wuxi (CN)
Assigned to Jiangnan University, Wuxi (CN)
Filed by Jiangnan University, Wuxi (CN)
Filed on Mar. 3, 2022, as Appl. No. 17/685,476.
Application 17/685,476 is a continuation of application No. PCT/CN2021/098057, filed on Jun. 3, 2021.
Claims priority of application No. 202010497171.5 (CN), filed on Jun. 4, 2020.
Prior Publication US 2022/0183974 A1, Jun. 16, 2022
Int. Cl. A61K 9/1278 (2025.01); A61K 8/14 (2006.01); A61K 31/375 (2006.01); A61P 3/02 (2006.01); A61Q 19/00 (2006.01)
CPC A61K 9/1278 (2013.01) [A61K 8/14 (2013.01); A61K 31/375 (2013.01); A61P 3/02 (2018.01); A61Q 19/00 (2013.01)] 7 Claims
OG exemplary drawing
 
1. A method for preparing fatty acid-based vitamin C (VC) liposomes, which comprises:
mixing food-grade linoleic acid and other fatty acids to make a mixture,
wherein condensed molecular formulas of the other fatty acids conform to a general formula CnH2n+1COOH, wherein n is a positive integer greater than 7,
wherein the food grade linoleic acid is a product obtained by alkali-catalyzed conjugation of food-grade industrial linoleic acid to make conjugated linoleic acid,
wherein the conjugated linoleic acid is a homogeneous substance or mixture of various octadecadienoic acid isomers, and
wherein the total octadecadienoic acid isomers account for 70% to 100% of a dry matter of the food grade linoleic acid by mass,
adding an sodium dodecyl sulfate (SDS) aqueous solution accounting for 5% to 10% of total fatty acids by mass into the mixture to create a complex of the linoleic acid and the other fatty acids with SDS to form a capsule material, wherein the capsule material is a self-assembled liquid, wherein a sum of final concentrations of fatty acids in the self-assembled liquid is 10 mM to 500 mM,
adjusting a pH of the self-assembled liquid to 3.5 to 4.0 with diluted acid,
homogenizing the self-assembled liquid to obtain a suspension
adding dropwise a VC aqueous solution while stirring into the suspension to embed VC thereby forming the fatty acid-based VC liposomes, and
shaking the mixture at 20 rpm to 50 rpm for 10 minutes to 1 hours.