US 12,268,774 B2
Compositions and methods for in utero delivery
W. Mark Saltzman, New Haven, CT (US); David H. Stitelman, Branford, CT (US); James Stephen Farrelly, Milford, CT (US); and Anthony Bianchi, Liverpool, NY (US)
Assigned to YALE UNIVERSITY, New Haven, CT (US)
Filed by Yale University, New Haven, CT (US)
Filed on Dec. 27, 2023, as Appl. No. 18/397,091.
Application 18/397,091 is a continuation of application No. 16/603,152, abandoned, previously published as PCT/US2018/026116, filed on Apr. 4, 2018.
Claims priority of provisional application 62/481,562, filed on Apr. 4, 2017.
Claims priority of provisional application 62/489,377, filed on Apr. 24, 2017.
Claims priority of provisional application 62/589,275, filed on Nov. 21, 2017.
Prior Publication US 2024/0207172 A1, Jun. 27, 2024
Int. Cl. A61K 9/00 (2006.01); A61K 9/16 (2006.01); A61K 9/51 (2006.01); A61K 38/18 (2006.01); A61K 47/60 (2017.01); A61K 48/00 (2006.01); A61P 7/00 (2006.01); A61P 11/00 (2006.01)
CPC A61K 9/0034 (2013.01) [A61K 9/0019 (2013.01); A61K 9/1647 (2013.01); A61K 9/1652 (2013.01); A61K 9/5153 (2013.01); A61K 38/1825 (2013.01); A61K 47/60 (2017.08); A61K 48/0041 (2013.01); A61P 7/00 (2018.01); A61P 11/00 (2018.01)] 13 Claims
 
1. A method comprising administering by injection into the amniotic fluid of a 15 to 20 week human embryo or fetus having exposed spinal cord or brain tissue, an effective amount of a composition comprising fibroblast growth factor encapsulated, entrapped, complexed to or dispersed in polymeric microparticles having a diameter from about 5 to about 10 microns and a zeta potential between −32.8 and −47 mV to cause epithelial tissue to form over the exposed spinal cord or brain tissue in the embryo or fetus, wherein the microparticles are formed of alginate, poly(lactic acid)(PLA), poly(glycolic acid)(PGA), poly(lactide-co-glycolide)(PLGA), polyanhydrides, poly(ortho) esters, poly(caprolactone), poly(hydroxyalkanoates), poly(lactide-co-caprolactone), poly(beta-amino) esters (PBAEs) or poly(amine-co-ester) polymers(PACE).