| CPC A61K 8/99 (2013.01) [A61K 8/64 (2013.01); A61Q 19/08 (2013.01)] | 16 Claims |
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1. An in vitro method for inducing fibroblast proliferation comprising contacting a preparation comprising fibroblasts with
(i) a microalgal culture supernatant obtained by a method comprising the steps of:
a) culturing microalgae belonging to Chlorophyceae class under conditions suitable for the growth of the microalgae and for the secretion of molecules to the culture medium, and
b) obtaining a microalgal culture supernatant by removing cells from the culture of step a), said cells maintaining the membrane integrity, and
c) concentrating the microalgal culture supernatant using a membrane with a cut off of at least 10 kDa by tangential flow filtration or by lyophilization followed by dialysis;
(ii) an IMAC-enriched microalgal culture supernatant obtained by a method comprising the steps of
a) culturing microalgae belonging to Chlorophyceae class under conditions suitable for the growth of the microalgae and for the secretion of molecules to the culture medium,
b) obtaining a microalgal culture supernatant by removing cells from the culture of step a), said cells maintaining the membrane integrity,
c) incubating the microalgal culture supernatant from step b) with a metal affinity chromatography resin under conditions allowing the binding of those molecules in the supernatant showing affinity to the resin,
d) separating the molecules that bind to the metal affinity chromatography resin from those that do not so bind and
e) eluting from the resin those molecules that bind to the resin, thereby obtaining an IMAC-enriched microalga culture supernatant, or a
(iii) a combination comprising a growth factor, and a microalgal culture supernatant as defined in (i) or an IMAC-enriched microalgal culture supernatant as defined in (ii).
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