US 12,268,727 B2
Bioactive polypeptides for improvements in plant protection, growth and productivity
Brian Thompson, Creve Coeur, MO (US); and Michelle Leslie, Webster Groves, MO (US)
Assigned to Spogen Biotech Inc., Saint Louis, MO (US)
Filed by Spogen Biotech Inc., St. Louis, MO (US)
Filed on Jun. 17, 2021, as Appl. No. 17/350,746.
Application 17/350,746 is a division of application No. 16/929,422, filed on Jul. 15, 2020, granted, now 11,046,735.
Application 16/929,422 is a division of application No. 16/041,059, filed on Jul. 20, 2018, granted, now 10,717,767, issued on Jul. 21, 2020.
Claims priority of provisional application 62/534,710, filed on Jul. 20, 2017.
Prior Publication US 2023/0016543 A1, Jan. 19, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 38/16 (2006.01); A01N 37/46 (2006.01); A01N 63/10 (2020.01); A01N 63/22 (2020.01); A01N 63/25 (2020.01); A01N 63/50 (2020.01); C07K 14/195 (2006.01); C07K 14/21 (2006.01); C07K 14/245 (2006.01); C07K 14/27 (2006.01); C07K 14/32 (2006.01); C12N 1/20 (2006.01); C12N 15/82 (2006.01); C12P 21/02 (2006.01)
CPC A61K 38/16 (2013.01) [A01N 37/46 (2013.01); A01N 63/10 (2020.01); A01N 63/50 (2020.01); C07K 14/195 (2013.01); C07K 14/21 (2013.01); C07K 14/245 (2013.01); C07K 14/27 (2013.01); C07K 14/32 (2013.01); C12N 1/20 (2013.01); C12N 15/82 (2013.01); C12P 21/02 (2013.01); A01N 63/22 (2020.01); A01N 63/25 (2020.01); C12N 2510/02 (2013.01)] 25 Claims
 
1. An isolated flagellin or flagellin-associated peptide for bioactive priming of a plant or a plant part to increase growth, yield, health, longevity, productivity, and/or vigor of a plant or a plant part and/or decrease abiotic stress in the plant or the plant part and/or protect the plant or the plant part from disease, insects and/or nematodes, and/or increase the innate immune response of the plant or the plant part and/or change plant architecture, wherein:
the peptide consists of the amino acid sequence SEQ ID NO: 752; and
a) arginine at amino acid position 1 of SEQ ID NO: 752 is optionally substituted with glutamine or lysine; or
b) serine at amino acid position 4 of SEQ ID NO: 752 is optionally substituted with glycine, threonine, asparagine, or arginine; or
c) lysine at amino acid position 6 of SEQ ID NO: 752 is optionally substituted with serine, alanine or glycine; or
d) aspartic acid at amino acid position 8 of SEQ ID NO: 752 is optionally substituted with proline; or
e) alanine at amino acid position 9 of SEQ ID NO: 752 is optionally substituted with proline; or
f) the flagellin or flagellin-associated peptide optionally has a chemical modification selected from the group consisting of acetylation, amidation, cyclization, and PEGylation, is part of a fusion protein wherein the fusion protein contains a protease recognition sequence, or a combination thereof; or
g) the peptide is optionally part of a fusion protein and the fusion protein has a signature peptide, wherein the amino acid sequence of the signature peptide has any one of SEQ ID NOs: 542-548, or any combination thereof; or a signal anchor sorting peptide, wherein the amino acid sequence of the signal anchor sorting peptide has any one of SEQ ID NOs: 549-562, or a combination thereof; or a secretion peptide, wherein the amino acid sequence of the secretion peptide has any one of SEQ ID NOs: 563-570 or 769.