US 12,268,719 B2
Bacteriophage composition and method of preventing bacterial infections in livestock
Rodrigue Dubar, Québec (CA); and Simon Labrie, Québec (CA)
Assigned to SYNTBIOLAB INC., Lévis (CA)
Filed by SYNTBIOLAB INC., Lévis (CA)
Filed on Oct. 24, 2023, as Appl. No. 18/493,396.
Application 18/493,396 is a continuation of application No. 16/647,445, granted, now 11,872,256, previously published as PCT/CA2018/051141, filed on Sep. 14, 2018.
Claims priority of provisional application 62/558,924, filed on Sep. 15, 2017.
Prior Publication US 2024/0082328 A1, Mar. 14, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 35/76 (2015.01); A23K 10/18 (2016.01); A61P 31/04 (2006.01); C12N 7/00 (2006.01)
CPC A61K 35/76 (2013.01) [A23K 10/18 (2016.05); A61P 31/04 (2018.01); C12N 7/00 (2013.01); C12N 2795/00032 (2013.01); C12N 2795/00051 (2013.01)] 20 Claims
 
1. A method for evaluating cross-resistance of at least two bacteriophage strains being screened for inclusion in a bacteriophage cocktail against a pathogenic bacteria, the method comprising:
providing a first and a second bacteriophage strain that infect the pathogenic bacteria, the second bacteriophage strain being different from the first bacteriophage strain;
culturing the first bacteriophage strain in the presence of said pathogenic bacteria at a Multiplicity of Infection (MOI) of at least about 1 until said bacteria develop resistance to the first bacteriophage strain, thereby producing a Bacteriophage Insensitive Mutant (BIM) pathogenic bacterial strain;
challenging the BIM pathogenic bacterial strain with the second bacteriophage strain; and
determining the absence of cross-resistance when the second bacteriophage strain still inhibits the growth of the BIM pathogenic bacterial strain.