US 12,268,207 B2
Systems and methods for extraction and cryopreservation of bone marrow
Erik J. Woods, Carmel, IN (US); Brian H. Johnstone, Fishers, IN (US); Dongsheng Gu, Indianapolis, IN (US); Aubrey Marie Sherry, Carmel, IN (US); and Kelsey Gwen Musall, Avon, IN (US)
Assigned to OSSIUM HEALTH, INC., San Francisco, CA (US)
Filed by OSSIUM HEALTH, INC., San Francisco, CA (US)
Filed on Jul. 14, 2023, as Appl. No. 18/352,942.
Application 18/352,942 is a continuation of application No. 17/684,259, filed on Mar. 1, 2022, granted, now 11,744,243.
Application 17/684,259 is a continuation of application No. PCT/US2021/055081, filed on Oct. 14, 2021.
Claims priority of provisional application 63/168,178, filed on Mar. 30, 2021.
Claims priority of provisional application 63/130,255, filed on Dec. 23, 2020.
Claims priority of provisional application 63/110,571, filed on Nov. 6, 2020.
Claims priority of provisional application 63/091,890, filed on Oct. 14, 2020.
Prior Publication US 2024/0164371 A1, May 23, 2024
Int. Cl. A01N 1/02 (2006.01)
CPC A01N 1/0284 (2013.01) [A01N 1/0221 (2013.01); A01N 1/0252 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method for processing a biological sample comprising cells or a derivative thereof, the method comprising:
(a) generating a first volume of the biological sample comprising cells or a derivative thereof, wherein the first volume comprises a first concentration of cells or a derivative thereof;
(b) generating a second volume of the biological sample comprising cells or a derivative thereof, wherein the second volume is less than the first volume and comprises a second concentration of the cells wherein the second concentration of the cells is no more than 30% different than the first concentration of the cells;
(c) generating a freezing curve specific for the cells;
(d) cooling the first volume at a first cooling rate, wherein the first cooling rate is generated from the freezing curve which balances damage from intracellular ice formation with damage from extracellular ice formation; and
(e) cooling the second volume at a second cooling rate, wherein the first cooling rate is generated from the freezing curve;
wherein the first cooling rate and second cooling rate are about the same and wherein a post-thaw cell proliferation rate of the cells in the first volume is no more than 30% different than a post-thaw cell proliferation rate of the cells in the second volume.